A herpes simplex virus 1 recombinant lacking the glycoprotein G coding sequences is defective in entry through apical surfaces of polarized epithelial cells in culture and in vivo.
A herpes simplex virus 1 recombinant lacking the glycoprotein G coding sequences is defective in entry through apical surfaces of polarized epithelial cells in culture and in vivo.
复制标题
缺乏糖蛋白G编码序列的单纯疱疹病毒1重组体在通过培养物和体内极化上皮细胞的顶面进入时存在缺陷。
DOI：
10.1073/pnas.020510297
复制
发表时间：
2000
影响因子：
11.1
通讯作者：
Sears,AE
中科院分区：
文献类型：
--
作者：
Tran,LC;Kissner,JM;Westerman,LE;Sears,AE
在感染新宿主的过程中，单纯疱疹病毒首先接触的表面是粘膜表面上皮细胞的顶膜。这些细胞是高度极化的，它们的顶膜和基底膜的蛋白质组成非常不同，因此每个表面都进化出不同的病毒进入途径。为了确定病毒糖蛋白G（gG）是否是有效感染极化细胞的特定表面所特异性需要的，用野生型病毒或gG缺失突变体感染顶端和基底表面。在感染培养物中的极化细胞后，gG−病毒缺乏顶端表面的感染，但能够通过基底膜感染细胞，复制并扩散到周围的细胞中。在根尖感染中的GG依赖性步骤是超越附着的阶段。在体内感染非划痕小鼠角膜上皮细胞的顶端表面后，与野生型病毒感染后观察到的感染相比，糖蛋白C−或gG−病毒的感染显著减少。相比之下，当角膜被划破，允许病毒进入其他细胞表面时，gG和糖蛋白C缺失突变体与野生型病毒一样有效地感染眼睛。在非极化细胞中病毒传代过程中，很容易出现允许gG−病毒感染顶端表面的继发突变，这表明可以绕过顶端感染的gG依赖性步骤，或者另一种病毒蛋白可以获得相同的功能。
During infection of a new host, the first surfaces encountered by herpes simplex viruses are the apical membranes of epithelial cells of mucosal surfaces. These cells are highly polarized, and the protein composition of their apical and basolateral membranes are very different, so that different viral entry pathways have evolved for each surface. To determine whether the viral glycoprotein G (gG) is specifically required for efficient infection of a particular surface of polarized cells, apical and basal surfaces were infected with wild-type virus or a gG deletion mutant. After infection of polarized cells in culture, the gG−virus was deficient in infection of apical surfaces but was able to infect cells through basal membranes, replicate, and spread into surrounding cells. The gG-dependent step in apical infection was a stage beyond attachment. Afterin vivoinfection of apical surfaces of epithelial cells of nonscarified mouse corneas, infection by glycoprotein C−or gG−virus was considerably reduced as compared with that observed after infection with wild-type virus. In contrast, when corneas were scarified, allowing virus access to other cell surfaces, the gG and glycoprotein C deletion mutants infected eyes as efficiently as wild-type viruses. A secondary mutation allowing infection of apical surfaces by gG−virus arose readily during passage of the virus in nonpolarized cells, indicating that either the gG-dependent step of apical infection can be bypassed or that another viral protein can acquire the same function.
登录
查看更多内容
影响因子：
3.8
作者：
HEROLD, BC;VISALLI, RJ;SPEAR, PG
通讯作者：
SPEAR, PG
影响因子：
5.4
作者：
JOHNSON, DC;LIGAS, MW
通讯作者：
LIGAS, MW
通过 Tn5 诱变快速鉴定 1 型单纯疱疹病毒非必需基因。
DOI：
10.1126/science.3033824
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Weber,PC;Levine,M;Glorioso,JC
通讯作者：
Glorioso,JC
影响通过活斑块的反应性鉴定的中和表位的构象或序列的突变与HSV-1(F) gB基因的syn和ts结构域分离。
影响因子：
3.7
作者：
Kousoulas,KG;Pellett,PE;Pereira,L;Roizman,B
通讯作者：
Roizman,B