Novel Models of Streptococcus canis Colonization and Disease Reveal Modest Contributions of M-Like (SCM) Protein.
Novel Models of Streptococcus canis Colonization and Disease Reveal Modest Contributions of M-Like (SCM) Protein.
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DOI：
10.3390/microorganisms9010183
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发表时间：
2021-01-16
期刊：
影响因子：
4.5
通讯作者：
Patras KA
中科院分区：
文献类型：
--
作者：
Cornax I;Zulk J;Olson J;Fulde M;Nizet V;Patras KA
犬链球菌是一种常见的猫和狗的泌尿生殖道定植细菌，也可引起这些动物种群和人类的侵袭性疾病。尽管犬链球菌的毒力机制尚不清楚，但一种m样蛋白SCM最近被确定为潜在的毒力因子。SCM是一种与宿主纤溶酶原和igg结合的表面相关蛋白，提示其在宿主-病原体相互作用中可能具有重要作用。在这项研究中，我们建立了犬链球菌阴道定植、全身感染和皮肤感染的体外和离体血液成分模型以及小鼠模型，以比较人畜共患犬链球菌阴道分离物G361及其等基因scm缺陷突变体（G361∆scm）的毒力潜力。我们发现，虽然犬链球菌在体内建立阴道定植并引起侵袭性疾病，但在这些模型中，SCM蛋白对毒力表型的贡献是适度的。我们的结论是，SCM对于小鼠模型中的侵袭性疾病和体外对人类血液成分的抗性是必不可少的，但可能有助于粘膜持久性，这突出了最近赏识的SCM在菌株和宿主之间的遗传多样性的潜在贡献。
Streptococcus canis is a common colonizing bacterium of the urogenital tract of cats and dogs that can also cause invasive disease in these animal populations and in humans. Although the virulence mechanisms of S. canis are not well-characterized, an M-like protein, SCM, has recently identified been as a potential virulence factor. SCM is a surface-associated protein that binds to host plasminogen and IgGs suggesting its possible importance in host-pathogen interactions. In this study, we developed in vitro and ex vivo blood component models and murine models of S. canis vaginal colonization, systemic infection, and dermal infection to compare the virulence potential of the zoonotic S. canis vaginal isolate G361 and its isogenic SCM-deficient mutant (G361∆scm). We found that while S. canis establishes vaginal colonization and causes invasive disease in vivo, the contribution of the SCM protein to virulence phenotypes in these models is modest. We conclude that SCM is dispensable for invasive disease in murine models and for resistance to human blood components ex vivo, but may contribute to mucosal persistence, highlighting a potential contribution to the recently appreciated genetic diversity of SCM across strains and hosts.
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DOI：
10.1128/genomea.00967-17
发表时间：
2017-09-21
期刊：
Genome announcements
影响因子：
--
作者：
Eichhorn I;van der Linden M;Jarek M;Fulde M
通讯作者：
Fulde M
影响因子：
3.6
作者：
Frick, IM;Mörgelin, M;Björck, L
通讯作者：
Björck, L
影响因子：
4.8
作者：
Anderson, Ericka L.;Cole, Jason N.;Nizet, Victor
通讯作者：
Nizet, Victor
影响因子：
9.4
作者：
Bert, F;LambertZechovsky, N
通讯作者：
LambertZechovsky, N
影响因子：
1.6
作者：
Enache, Andra E.;Mitchell, Catriona;Waller, Andrew S.
通讯作者：
Waller, Andrew S.