Host-Species Variation and Environment Influence Endophyte Symbiosis and Mycotoxin Levels in Chinese Oxytropis Species.
Host-Species Variation and Environment Influence Endophyte Symbiosis and Mycotoxin Levels in Chinese Oxytropis Species.
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DOI：
10.3390/toxins14030181
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发表时间：
2022-02-28
期刊：
影响因子：
4.2
通讯作者：
He W
中科院分区：
文献类型：
--
作者：
Guo C;Zhang L;Zhao Q;Beckmann M;Phillips H;Meng H;Mo C;Mur LAJ;He W
棘豆属植物广泛分布于中国北方草原。据报道，一些棘豆属物种含有真菌毒素苦马豆素，这是一种引起牲畜中毒的生物碱，被称为地方中毒。以前的研究表明，内生真菌（oxytropis Alternaria）与这些oxytropis物种共生，产生马豆素。然而，对棘豆属内变异对共生关系的固定或丧失和毒性的影响知之甚少，环境因素的影响也是如此。本研究以中国北方地区17种棘豆属植物为研究对象，采用基因分型法对其遗传多样性进行了分析，并与内生菌和马豆素水平进行了比较。结果表明，9种棘豆属昆虫有可检测到的棘豆属定植，7种棘豆属昆虫含有足以被认为有毒的马豆素，而其余的棘豆属昆虫可能无毒。物种变异而不是遗传谱系与内生菌和苦豆素生产的固定或丧失有关，这似乎是遗传漂变的结果。基因型×环境（G × E）效应也会影响棘豆属植物的内生菌和苦豆素水平。本研究将为更好地理解棘豆草共生的进化基础和麻豆素在麻豆草中的生物合成提供依据。
Oxytropis plants are widely distributed in the grasslands in northern China. Some Oxytropis species have been reported to contain the mycotoxin swainsonine, an alkaloid which causes poisoning in livestock, referred to as locoism. Previous studies showed that endophytic fungi (Alternaria oxytropis) symbiotically associate with these Oxytropis species to produce swainsonine. However, the influence of variation within the Oxytropis genus on the fixation or loss of symbiosis and toxicity is poorly understood, as is the influence of environmental factors. Here we used a collection of 17 common Oxytropis species sampled in northern China to assess genetic diversity using genotyping by sequencing which was compared with the levels of the endophyte and swainsonine. Results showed that nine Oxytropis species have detectable A. oxytropis colonisation, and seven Oxytropis species contain sufficient swainsonine to be considered poisonous, whereas the rest may be non-toxic. Species variation rather than the genetic lineage was associated with the fixation or loss of endophyte and swainsonine production, which appears to have resulted from genetic drift. Genotype × Environment (G × E) effects were also found to influence endophyte and swainsonine levels amongst species of the Oxytropis genus. Our study will provide a better understanding about the evolutionary basis of A. oxytropis symbiosis and swainsonine biosynthesis in locoweeds.
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影响因子：
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