Real-time observation of G-quadruplex dynamics using single-molecule FRET microscopy.
Real-time observation of G-quadruplex dynamics using single-molecule FRET microscopy.
复制标题
使用单分子 FRET 显微镜实时观察 G-四链体动力学。
DOI：
10.1007/978-1-59745-363-9_6
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ha,Taekjip
中科院分区：
文献类型：
--
作者：
Okumus,Burak;Ha,Taekjip
The potential importance of G-quadruplex structures was implied by the recent findings that the human POT1 disrupts G-quadruplex and stimulates the telomerase activity. A solid understanding of the range of conformations that can be adopted by guanine-rich sequences can potentially shed much light on the molecular mechanisms underlying certain human diseases related to telomeres. Furthermore, structure-based design of chemotherapeutic drugs for cancer might be realized by addressing different types of G-quadruplex structures. Using the unique capabilities of single-molecule spectroscopy, we have recently reported on the intricate dynamic structural properties of a minimal form of human telomeric DNA. Here, we present the detailed step-by-step methods for the real-time observation of G-rich DNA sequences by means of single-molecule FRET microscopy and provide the protocols for vesicle encapsulation and surface immobilization assays. Such assays provide a firm basis for future studies aimed at elucidating the interaction between telomeric DNA and telomere-associated proteins as well as the synthetic therapeutic agents that specifically stabilize certain G-quadruplex topologies.
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DOI：
--
发表时间：
1976
期刊：
Journal of the American Medical Association (JAMA)
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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DOI：
10.1016/0002-9149(84)90646-5
发表时间：
1984
期刊：
The American journal of cardiology
影响因子：
--
作者：
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