Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11
Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11
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Hfq 调节溶杆菌 OH11 中抗菌抗生素的生物合成和细胞外裂解酶的产生
DOI：
10.1111/1751-7915.12246
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发表时间：
2015-02
影响因子：
5.7
通讯作者：
Liu Fengquan
中科院分区：
文献类型：
--
作者：
Xu Gaoge;Zhao Yuxin;Du Liangcheng;Qian Guoliang;Liu Fengquan
产酶溶杆菌是一种重要的生防菌，能产生多种溶酶和新型抗生素。对它们的调控机制知之甚少。了解这些特性将有助于提高作物病害的生物防治水平和潜在的医学应用。在本研究中，我们产生了一个hfq（编码假定的核糖核酸伴侣）缺失突变体，然后利用一种新的无基因组标记的方法来构建一个hfq互补菌株。首次证明了Hfq在产酶乳杆菌抗菌抗生素生物合成和胞外裂解酶活性调节中具有多效性。hfq的突变显著增加了WAP-8294 A2（一种抗菌抗生素）的产量及其关键生物合成基因waps 1的转录。而hfq的失活几乎使产酶乳杆菌的胞外几丁质酶活性丧失，胞外蛋白酶和纤维素酶活性显著降低。我们进一步表明，hfq对胞外几丁质酶产生的调节部分是通过抑制几丁质酶A的分泌来实现的。总的来说，我们的研究结果揭示了hfq在抗生素代谢产物和胞外溶解酶中的调节作用。
Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker‐free method to construct an hfq‐complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial antibiotic biosynthesis and extracellular lytic enzyme activity in L. enzymogenes. Mutation of hfq significantly increased the yield of WAP‐8294A2 (an antibacterial antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L. enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in antibiotic metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.
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影响因子：
3.7
作者：
Kadzhaev K;Zingmark C;Golovliov I;Bolanowski M;Shen H;Conlan W;Sjöstedt A
通讯作者：
Sjöstedt A
影响因子：
4.4
作者：
Qian, Guoliang;Wang, Yulan;Liu, Fengquan
通讯作者：
Liu, Fengquan
影响因子：
3.2
作者：
Kharade, Sampada S.;McBride, Mark J.
通讯作者：
McBride, Mark J.
影响因子：
3.7
作者：
Ansong C;Yoon H;Porwollik S;Mottaz-Brewer H;Petritis BO;Jaitly N;Adkins JN;McClelland M;Heffron F;Smith RD
通讯作者：
Smith RD
影响因子：
14.9
作者：
KAJITANI, M;ISHIHAMA, A
通讯作者：
ISHIHAMA, A