The Cape Verde Islands Allele of Cryptochrome 2 Enhances Cotyledon Unfolding in the Absence of Blue Light in Arabidopsis1[w]
The Cape Verde Islands Allele of Cryptochrome 2 Enhances Cotyledon Unfolding in the Absence of Blue Light in Arabidopsis1[w]
复制标题
佛得角群岛隐花色素 2 等位基因在没有蓝光的情况下增强拟南芥子叶的展开1[w]
作者：
J. Botto;C. Alonso‐Blanco;Ignacio Garzarón;R. Sánchez;J. Casal
通过对105个重组自交系的研究，分析了Landsburg erecta(LER)和佛得角群岛(CVI)材料之间的自然遗传变异，以寻找在拟南芥黄化幼苗中光敏色素感知的光信号敏感性调节中的成员。在每小时远红光脉冲下生长的幼苗中，我们确定了3个控制下胚轴生长的QTL(VLF3、VLF4和VLF5)和3个不同的控制子叶展开的QTL(VLF6、VLF7和VLF1)。这表明不同的生理输出对脱黄化过程中的敏感性有选择性的调节。与CVI等位基因相比，VLF3、VLF4、VLF5、VLF7和VLF1的LER等位基因增强，而VLF6的LER等位基因降低，对FR脉冲的反应。利用近等基因系确认并缩小了部分QTL的定位范围。VLF6定位于CRY2(隐花色素2)基因附近。与野生型或表达CRY2-LER等位基因的转基因植株相比，表达CRY2-LER等位基因的转基因LER幼苗在每小时的FR脉冲下表现出更强的子叶展开能力。这种反应需要光敏色素A。缺乏这两种隐色素的Cry1-Cry2双突变体在FR脉冲下表现为子叶展开减少。由于CRY2-CVI与CRY2-LER相比是一个功能增益等位基因，因此隐花色素活性与FR脉冲下子叶的展开呈正相关。我们认为，蓝光感受器隐花色素2在没有蓝光的情况下可以调节幼苗的光形态建成。除了核基因外，我们还发现了细胞质对幼苗去黄化的影响。
We analyzed the natural genetic variation between Landsburg erecta (Ler) and Cape Verde Islands (Cvi) accessions by studying 105 recombinant inbred lines to search for players in the regulation of sensitivity to light signals perceived by phytochromes in etiolated seedlings of Arabidopsis. In seedlings grown under hourly pulses of far-red (FR) light, we identified three quantitative trait loci (QTLs; VLF3, VLF4, and VLF5) for hypocotyl growth inhibition and three different QTLs (VLF6, VLF7, and VLF1) for cotyledon unfolding. This indicates that different physiological outputs have selective regulation of sensitivity during de-etiolation. Ler alleles, compared with Cvi alleles, of VLF3, VLF4, VLF5, VLF7, and VLF1 enhanced, whereas the Ler allele of VLF6 reduced, the response to pulses of FR. We confirmed and narrowed down the position of some QTLs by using near-isogenic lines. VLF6 mapped close to the CRY2 (cryptochrome 2) gene. Transgenic Ler seedlings expressing the Cvi allele of CRY2 showed enhanced cotyledon unfolding under hourly pulses of FR compared with the wild type or transgenics expressing the CRY2-Ler allele. This response required phytochrome A. The cry1 cry2 double mutant lacking both cryptochromes showed reduced cotyledon unfolding under FR pulses. Because the CRY2-Cvi is a gain-of-function allele compared with CRY2-Ler, cryptochrome activity correlates positively with cotyledon unfolding under FR pulses. We conclude that the blue light photoreceptor cryptochrome 2 can modulate seedling photomorphogenesis in the absence of blue light. In addition to the nuclear loci, we identified cytoplasmic effects on seedling de-etiolation.
影响因子：
3.3
作者：
J. Borevitz;J. Maloof;J. Lutes;T. Dabi;Joanna L. Redfern;Gabriel T. Trainer;Jonathan D. Werner;T. Asami;C. Berry;D. Weigel;J. Chory
通讯作者：
J. Borevitz;J. Maloof;J. Lutes;T. Dabi;Joanna L. Redfern;Gabriel T. Trainer;Jonathan D. Werner;T. Asami;C. Berry;D. Weigel;J. Chory
DOI：
10.1073/pnas.95.5.2686
发表时间：
1998-03-03
影响因子：
11.1
作者：
Lin, CT;Yang, HY;Cashmore, AR
通讯作者：
Cashmore, AR
影响因子：
10.5
作者：
Moller, SG;Kunkel, T;Chua, NH
通讯作者：
Chua, NH