Genomic convergence analysis of schizophrenia: mRNA sequencing reveals altered synaptic vesicular transport in post-mortem cerebellum.
Genomic convergence analysis of schizophrenia: mRNA sequencing reveals altered synaptic vesicular transport in post-mortem cerebellum.
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DOI：
10.1371/journal.pone.0003625
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Kingsmore, Stephen F.
中科院分区：
文献类型：
--
作者：
Mudge, Joann;Miller, Neil A.;Khrebtukova, Irina;Lindquist, Ingrid E.;May, Gregory D.;Huntley, Jim J.;Luo, Shujun;Zhang, Lu;van Velkinburgh, Jennifer C.;Farmer, Andrew D.;Lewis, Sharon;Beavis, William D.;Schilkey, Faye D.;Virk, Selene M.;Black, C. Forrest;Myers, M. Kathy;Mader, Lar C.;Langley, Ray J.;Utsey, John P.;Kim, Ryan W.;Roberts, Rosalinda C.;Khalsa, Sat Kirpal;Garcia, Meredith;Ambriz-Griffith, Victoria;Harlan, Richard;Czika, Wendy;Martin, Stanton;Wolfinger, Russell D.;Perrone-Bizzozero, Nora I.;Schroth, Gary P.;Kingsmore, Stephen F.
精神分裂症（SCZ）是一种常见的致残性精神疾病，具有高遗传性，但复杂，遗传病因学知之甚少。作为SCZ基因组聚合分析的第一阶段，我们从14名患者和6名匹配对照的死后小脑皮质中产生了167亿个核苷酸的短读段、鸟枪序列的cDNA。开发了一个严格的分析管道，用于分析数字基因表达研究。每个样品中的序列与大约33，200个转录物比对，每个基因的平均覆盖率为450个读数。在对混杂的临床、样本和实验变异来源进行调整后，病例和对照组之间有215个基因的表达存在显著差异。高尔基体、囊泡运输、膜结合、锌结合和转录调控等基因在差异表达基因中占优势。23个基因的表达改变，参与突触前囊泡运输，高尔基体功能和GABA能神经传递定义了一个统一的分子假说，在SCZ小脑皮质功能障碍。
Schizophrenia (SCZ) is a common, disabling mental illness with high heritability but complex, poorly understood genetic etiology. As the first phase of a genomic convergence analysis of SCZ, we generated 16.7 billion nucleotides of short read, shotgun sequences of cDNA from post-mortem cerebellar cortices of 14 patients and six, matched controls. A rigorous analysis pipeline was developed for analysis of digital gene expression studies. Sequences aligned to approximately 33,200 transcripts in each sample, with average coverage of 450 reads per gene. Following adjustments for confounding clinical, sample and experimental sources of variation, 215 genes differed significantly in expression between cases and controls. Golgi apparatus, vesicular transport, membrane association, Zinc binding and regulation of transcription were over-represented among differentially expressed genes. Twenty three genes with altered expression and involvement in presynaptic vesicular transport, Golgi function and GABAergic neurotransmission define a unifying molecular hypothesis for dysfunction in cerebellar cortex in SCZ.
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影响因子：
21.3
作者：
Haas, AK;Fuchs, E;Barr, FA
通讯作者：
Barr, FA
影响因子：
46.9
作者：
Canales, Roger D.;Luo, Yuling;Goodsaid, Federico M.
通讯作者：
Goodsaid, Federico M.
DOI：
10.1098/rspb.2007.0876
发表时间：
2007-11-22
影响因子：
4.7
作者：
Crespi, Bernard;Summers, Kyle;Dorus, Steve
通讯作者：
Dorus, Steve
影响因子：
6.1
作者：
Evans, SJ;Choudary, PV;Akil, H
通讯作者：
Akil, H
影响因子：
30.8
作者：
Allen, Nicole C.;Bagade, Sachin;Bertram, Lars
通讯作者：
Bertram, Lars