Bluetongue virus assembly and exit pathways.
Bluetongue virus assembly and exit pathways.
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DOI：
10.1016/bs.aivir.2020.08.002
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发表时间：
2020
影响因子：
--
通讯作者：
Roy P
中科院分区：
文献类型：
--
作者：
Roy P
蓝舌病毒(Bluetongue Virus，BTV)是世界上许多地区野生反刍动物和家畜的一种由昆虫传播的新病原体。病毒粒子是由连续的蛋白质层组成的复杂结构，环绕着10个双链(DS)RNA片段的基因组。BTV作为一种大的、无包膜的dsRNA病毒的模型系统已被广泛研究。重组蛋白质和颗粒与反向遗传学、X射线结晶学高分辨率结构分析和冷冻电子显微镜技术相结合，为衣壳的组装和所需的蛋白质隔离提供了顺序。此外，重组的体外组装系统和RNA-RNA相互作用实验定义了组装和包装10段RNA基因组所需的各个步骤。此外，各种显微技术已经被用来阐明病毒成熟的阶段及其通过多种途径流出。这些发现不仅对BTV的组装和形态发生有了全面的了解，而且表明类似的组装和出口途径可能被相关病毒利用，并为干预或预防提供了一个知情的起点。
Bluetongue virus (BTV) is an insect-vectored emerging pathogen of wild ruminants and livestock in many parts of the world. The virion particle is a complex structure of consecutive layers of protein surrounding a genome of ten double-stranded (ds) RNA segments. BTV has been studied extensively as a model system for large, non-enveloped dsRNA viruses. A combination of recombinant proteins and particles together with reverse genetics, high-resolution structural analysis by X-ray crystallography and cryo-electron microscopy techniques have been utilized to provide an order for the assembly of the capsid shell and the protein sequestration required for it. Further, a reconstituted in vitro assembly system and RNA-RNA interaction assay, have defined the individual steps required for the assembly and packaging of the 10-segmented RNA genome. In addition, various microscopic techniques have been utilised to illuminate the stages of virus maturation and its egress via multiple pathways. These findings have not only given an overall understanding of BTV assembly and morphogenesis but also indicated that similar assembly and egress pathways are likely to be used by related viruses and provided an informed starting point for intervention or prevention.
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影响因子：
6.7
作者：
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通讯作者：
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影响因子：
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作者：
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