Synthesis of galactofuranose-based acceptor substrates for the study of the carbohydrate polymerase GlfT2.
Synthesis of galactofuranose-based acceptor substrates for the study of the carbohydrate polymerase GlfT2.
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DOI：
10.1016/j.bmc.2010.04.068
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发表时间：
2010-06-01
影响因子：
3.5
通讯作者：
Kiessling, Laura L.
中科院分区：
文献类型：
--
作者：
Splain, Rebecca A.;Kiessling, Laura L.
关键词：
Despite the prevalence and importance of carbohydrate polymers, the molecular details of their biosynthesis remain elusive. Many enzymes responsible for the synthesis of carbohydrate polymers require a “primer” or “initiator” carbohydrate sequence. One example of such an enzyme is the mycobacterial galactofuranosyltransferase GlfT2 (Rv3808c), which generates an essential cell wall building block. We recently demonstrated that recombinant GlfT2 is capable of producing a polymer composed of alternating β-(1,5) and β-(1,6)-linked galactofuranose (Galf) residues. Intriguingly, the length of the polymers produced from a synthetic glycosyl acceptor is consistent with those found in the cell wall. To probe the mechanism by which polymer length is controlled, a collection of initiator substrates has been assembled. The central feature of the synthetic route is a ruthenium-catalyzed cross-metathesis as the penultimate transformation. Access to synthetic substrates has led us to postulate a new mechanism for length control in this template independent polymerization. Moreover, our investigations indicate that lipids possessing but a single galactofuranose residue can act as a substrate for GlfT2.
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