Strigolactone regulation of shoot branching in chrysanthemum (Dendranthema grandiflorum).
Strigolactone regulation of shoot branching in chrysanthemum (Dendranthema grandiflorum).
复制标题
DOI：
10.1093/jxb/erq133
复制
发表时间：
2010-06
影响因子：
6.9
通讯作者：
Leyser O
中科院分区：
文献类型：
--
作者：
Liang J;Zhao L;Challis R;Leyser O
先前对豌豆（rms 1-rms 5）、拟南芥（max 1-max 4）、矮牵牛（dad 1-dad 3）和水稻（d3、d10、htd 1/d17、d14、d27）的高度分支突变体的研究鉴定了独脚金内酯或其衍生物（SL）作为芽分支抑制剂。最近的这一发现提供了使用SL在商业上调节分支的可能性，例如，在菊花中，一种重要的切花作物。为了研究这一选择，研究了菊花（Dendranthema grandiflorum）的SL生理和分子生物学，重点是CCD 8/MAX 4/DAD 1/RMS 1/D10基因。我们的研究结果表明，已经提出的拟南芥，SL抑制芽活性的能力取决于竞争生长素源的存在。克隆了菊花SL生物合成基因CCD 8，发现其以与已知CCD 8相似但不相同的方式进行调控。表达分析表明，DgCCD 8主要在根和茎中表达，并被外源生长素上调。外源SL可以下调DgCCD 8的表达，但这种效果可以被顶端生长素的应用所覆盖。这项研究提供了证据，表明SL是改变菊花枝条分支习惯的有希望的候选者。
Previous studies of highly branched mutants in pea (rms1–rms5), Arabidopsis thaliana (max1–max4), petunia (dad1–dad3), and rice (d3, d10, htd1/d17, d14, d27) identified strigolactones or their derivates (SLs), as shoot branching inhibitors. This recent discovery offers the possibility of using SLs to regulate branching commercially, for example, in chrysanthemum, an important cut flower crop. To investigate this option, SL physiology and molecular biology were studied in chrysanthemum (Dendranthema grandiflorum), focusing on the CCD8/MAX4/DAD1/RMS1/D10 gene. Our results suggest that, as has been proposed for Arabidopsis, the ability of SLs to inhibit bud activity depends on the presence of a competing auxin source. The chrysanthemum SL biosynthesis gene, CCD8 was cloned, and found to be regulated in a similar, but not identical way to known CCD8s. Expression analyses revealed that DgCCD8 is predominantly expressed in roots and stems, and is up-regulated by exogenous auxin. Exogenous SL can down-regulate DgCCD8 expression, but this effect can be overridden by apical auxin application. This study provides evidence that SLs are promising candidates to alter the shoot branching habit of chrysanthemum.
登录
查看更多内容
影响因子：
7.4
作者：
Dun, Elizabeth Ann;Ferguson, Brett James;Beveridge, Christine Anne
通讯作者：
Beveridge, Christine Anne
影响因子：
7.4
作者：
Morris, SE;Turnbull, CGN;Beveridge, CA
通讯作者：
Beveridge, CA
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
9.2
作者：
Booker, J;Auldridge, M;Leyser, O
通讯作者：
Leyser, O
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF