Selective cytotoxicity of aids virus infection towards HTLV‐I‐transformed cell lines
Selective cytotoxicity of aids virus infection towards HTLV‐I‐transformed cell lines
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艾滋病病毒感染对 HTLV-I 转化细胞系的选择性细胞毒性
DOI：
10.1002/ijc.2910360406
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发表时间：
1985
影响因子：
6.4
通讯作者：
F. Uchino
中科院分区：
文献类型：
--
作者：
Y. Koyanagi;S. Harada;N. Yamamoto;Mutsuo Takahashi;F. Uchino
以前，我们报道了人类T细胞嗜淋巴细胞病毒1型（HTLV-I）转化系MT-2和MT-4的细胞被艾滋病逆转录病毒HTLY-III感染广泛杀死。我们已经研究了这种现象更系统地使用光学和电子显微镜以及免疫荧光。本研究中使用的细胞系包括14种不仅携带人HTLY-1而且携带相关猿猴因子的细胞系和6种HTLY-I-阴性T-和B-细胞系。结果表明，杀细胞作用仅发生在HTLY‐I‐转化细胞系中，并且在进一步的传代培养中不存在。在这些细胞系中，细胞毒性反应与病毒感染后HTLV‐III抗原的诱导密切相关。然而，6个无HTLV-I-细胞系的细胞并未被HTLV-III在明显程度上杀死，并作为艾滋病病毒的连续生产者进行传代。在14种携带HTLV-I的细胞系中，仅2种细胞系对HTLV-III的杀细胞作用具有抗性。它们也对感染的HTLV-III的复制具有抗性。在HTLV‐I转化细胞系中观察到的这种AIDS病毒特异性细胞毒性效应似乎与HTLV‐I基因组的gag和pXs区域的基因表达无关。这一结果可能表明HTLV-III特异性干扰HTLV-I转化的某些步骤。
Previously, we reported that cells of the human T‐cell lymphotropic virus type 1 (HTLV‐I)‐transformed lines MT‐2 and MT‐4 were extensively killed by infection with AIDS retrovirus HTLY‐III. We have investigated this phenomenon more systematically using light and electron microscopy as well as immunofluorescence. The cell lines used in the present studies included 14 of those carrying not only human HTLY‐1 but also related simian agents and 6 HTLY‐I‐negative T‐ and B‐cell lines. The results showed that the cytocidal effects occurred in the HTLY‐I‐transformed cell lines exclusively and were not present in further subcultures. In these cell lines the cytotoxic response was closely correlated with the induction of HTLV‐III antigens after virus infection. However, cells of 6 HTLV‐I‐free lines were not killed to a marked extent by HTLV‐III and were passaged as continuous producers of AIDS virus. Only 2 cell lines were resistant to the cytocidal effect of HTLV‐III among 14 HTLV‐I carrying cell lines. They were also resistant to the replication of infected HTLV‐III. This AIDS virus‐specific cytotoxic effect observed in HTLV‐I‐transformed cell lines did not appear to be associated with gene expression of the gag and pXs region of HTLV‐I genomes. This result may indicate that HTLV‐III specifically interferes with some steps of HTLV‐I transformation.
影响因子：
56.9
作者：
SODROSKI, J;ROSEN, C;HASELTINE, WA
通讯作者：
HASELTINE, WA