Identification of miRNA-mRNA Crosstalk in Respiratory Syncytial Virus- (RSV-) Associated Pediatric Pneumonia through Integrated miRNAome and Transcriptome Analysis
Identification of miRNA-mRNA Crosstalk in Respiratory Syncytial Virus- (RSV-) Associated Pediatric Pneumonia through Integrated miRNAome and Transcriptome Analysis
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通过整合 miRNA 组和转录组分析鉴定呼吸道合胞病毒 (RSV-) 相关小儿肺炎中的 miRNA-mRNA 串扰
DOI：
10.1155/2020/8919534
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发表时间：
2020-05
影响因子：
4.6
通讯作者：
Lu Gen
中科院分区：
文献类型：
--
作者：
Zhang Xu;Huang Feng;Yang Diyuan;Peng Tao;Lu Gen
Respiratory syncytial virus (RSV) is the most common respiratory virus and is associated with pediatric pneumonia, causing bronchiolitis and significant mortality in infants and young children. MicroRNAs (miRNAs) are endogenous noncoding small RNAs that function in gene regulation and are associated with host immune response and disease progression. In the present study, we profiled the global transcriptome and miRNAome of whole blood samples from children with mild or severe RSV-associated pneumonia, aiming to identify the potential biomarkers and investigate the molecular mechanisms of severe RSV-associated pediatric pneumonia. We found that expression profiles of whole blood microRNAs and mRNAs were altered and distinctly different in children with severe RSV-associated pneumonia. In particular, the four most significantly upregulated miRNAs in children with severe RSV-associated pneumonia were hsa-miR-1271-5p, hsa-miR-10a-3p, hsa-miR-125b-5p, and hsa-miR-30b-3p. The severe RSV-associated pneumonia-specific differentially expressed miRNA target interaction network was also contrasted. These target genes were further analyzed with Gene Ontology enrichment analysis. We found that most of the target genes were involved in inflammatory and immune responses, including the NF-κB signaling pathway, the MAPK signaling pathway, and T cell receptor signaling. Our findings will contribute to the identification of biomarkers and new drug design strategies to treat severe RSV-associated pediatric pneumonia.
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影响因子：
168.9
作者：
Bhutta, Zulfiqar A.;Das, Jai K.;Black, Robert E.
通讯作者：
Black, Robert E.
DOI：
10.1111/tmi.12368
发表时间：
2014-11
期刊：
Tropical medicine & international health : TM & IH
影响因子：
--
作者：
Agweyu A;Kibore M;Digolo L;Kosgei C;Maina V;Mugane S;Muma S;Wachira J;Waiyego M;Maleche-Obimbo E
通讯作者：
Maleche-Obimbo E
DOI：
10.1016/s2214-109x(17)30344-3
发表时间：
2017-10
期刊：
The Lancet. Global health
影响因子：
--
作者：
Scheltema NM;Gentile A;Lucion F;Nokes DJ;Munywoki PK;Madhi SA;Groome MJ;Cohen C;Moyes J;Thorburn K;Thamthitiwat S;Oshitani H;Lupisan SP;Gordon A;Sánchez JF;O'Brien KL;PERCH Study Group;Gessner BD;Sutanto A;Mejias A;Ramilo O;Khuri-Bulos N;Halasa N;de-Paris F;Pires MR;Spaeder MC;Paes BA;Simões EAF;Leung TF;da Costa Oliveira MT;de Freitas Lázaro Emediato CC;Bassat Q;Butt W;Chi H;Aamir UB;Ali A;Lucero MG;Fasce RA;Lopez O;Rath BA;Polack FP;Papenburg J;Roglić S;Ito H;Goka EA;Grobbee DE;Nair H;Bont LJ
通讯作者：
Bont LJ
影响因子：
11.8
作者：
Al-Sonboli N;Hart CA;Al-Aghbari N;Al-Ansi A;Ashoor O;Cuevas LE
通讯作者：
Cuevas LE
影响因子：
100.3
作者：
Qiutang Li;I. Verma
通讯作者：
Qiutang Li;I. Verma