Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis.
Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis.
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DOI：
10.1073/pnas.2021970118
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发表时间：
2021-08-17
影响因子：
11.1
通讯作者：
Ziolkowski PA
中科院分区：
文献类型：
--
作者：
Zhu L;Fernández-Jiménez N;Szymanska-Lejman M;Pelé A;Underwood CJ;Serra H;Lambing C;Dluzewska J;Bieluszewski T;Pradillo M;Henderson IR;Ziolkowski PA
减数分裂重组在真核生物遗传多样性的形成中起着重要作用。在种群和种之间存在着广泛的交叉率差异。修饰位点的身份及其在基因组进化中的作用仍不完全清楚。我们探索了拟南芥杂交的自然变异，并确定了SNI1是一个主要修饰位点的致病基因。迄今为止，SNI1在交叉中没有已知的作用。SNI1是SMC5/6复合体的一个组成部分，与黏结蛋白和凝缩蛋白密切相关。拟南芥sni1和其他SMC5/6突变体在不受干扰的交叉通路上表现出类似的作用。因此，我们的研究结果表明，SMC5/6复合体在DNA损伤修复中起着重要作用，对减数分裂交叉的控制也很重要。减数分裂交叉的频率和分布受到严格控制；然而，在这一过程中可以观察到物种内部和物种之间的差异。利用两个天然拟南芥材料Col和Ler的杂交，我们将一个交叉修饰位点定位到编码SMC5/6复合体成分的NPR1-1 INDUCIBLE 1 (SNI1) SUPPRESSOR的半显性多态性上。sni1突变体在整个基因组中表现出一种修饰的重组模式，染色体远端区域的交叉率升高，而中心点周围区域的交叉率降低。SNI1的突变导致交叉干扰减少，可以部分恢复I类交叉途径突变体的生育能力，这表明该蛋白影响非干扰交叉修复。因此，我们测试了SNI1与RECQ4和FANCM DNA解旋酶之间的遗传相互作用，结果表明，在SNI1突变体中观察到的额外II类交叉与FANCM无关。此外，对其他SMC5/6突变体的遗传分析证实了SNI1的交叉再分布。该研究揭示了SMC5/6复合体在保证植物减数分裂重组正常进行中的重要性。
Meiotic recombination plays a fundamental role in shaping genetic diversity in eukaryotes. Extensive variation in crossover rate exists between populations and species. The identity of modifier loci and their roles in genome evolution remain incompletely understood. We explored natural variation in Arabidopsis crossover and identified SNI1 as the causal gene underlying a major modifier locus. To date, SNI1 had no known role in crossover. SNI1 is a component of the SMC5/6 complex that is closely related to cohesin and condensin. Arabidopsis sni1 and other SMC5/6 mutants show similar effects on the interference-independent crossover pathway. Hence, our findings demonstrate that the SMC5/6 complex, which is known for its role in DNA damage repair, is also important for control of meiotic crossover. The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphisms in SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), which encodes a component of the SMC5/6 complex. The sni1 mutant exhibits a modified pattern of recombination across the genome with crossovers elevated in chromosome distal regions but reduced in pericentromeres. Mutations in SNI1 result in reduced crossover interference and can partially restore the fertility of a Class I crossover pathway mutant, which suggests that the protein affects noninterfering crossover repair. Therefore, we tested genetic interactions between SNI1 and both RECQ4 and FANCM DNA helicases, which showed that additional Class II crossovers observed in the sni1 mutant are FANCM independent. Furthermore, genetic analysis of other SMC5/6 mutants confirms the observations of crossover redistribution made for SNI1. The study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants.
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影响因子：
4.5
作者：
Choi K;Reinhard C;Serra H;Ziolkowski PA;Underwood CJ;Zhao X;Hardcastle TJ;Yelina NE;Griffin C;Jackson M;Mézard C;McVean G;Copenhaver GP;Henderson IR
通讯作者：
Henderson IR
影响因子：
3.5
作者：
Diaz M;Pecinka A
通讯作者：
Pecinka A
影响因子：
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作者：
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通讯作者：
Pillen, Klaus
DOI：
10.15252/embj.2020104858
发表时间：
2020-11-02
期刊：
The EMBO journal
影响因子：
--
作者：
Blackwell AR;Dluzewska J;Szymanska-Lejman M;Desjardins S;Tock AJ;Kbiri N;Lambing C;Lawrence EJ;Bieluszewski T;Rowan B;Higgins JD;Ziolkowski PA;Henderson IR
通讯作者：
Henderson IR
影响因子：
56.9
作者：
Crismani, Wayne;Girard, Chloe;Mercier, Raphael
通讯作者：
Mercier, Raphael