Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes.
Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes.
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DOI：
10.1002/pmic.201700156
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发表时间：
2017-10
期刊：
影响因子：
3.4
通讯作者：
Kelleher NL
中科院分区：
文献类型：
--
作者：
Haverland NA;Waas M;Ntai I;Keppel T;Gundry RL;Kelleher NL
淋巴细胞是维持获得性免疫的关键免疫细胞。淋巴祖细胞的分化产生B、T和NK细胞亚型，这些亚型分别与特定形式的白血病或淋巴瘤相关。因此，利用一种精确的细胞分类方法来检测患者不同疾病状态的差异是非常必要的。一种可行的分类方法包括评估淋巴系恶性肿瘤的细胞表面蛋白质组。具体地说，这篇手稿详细介绍了使用一种称为细胞表面捕获技术的抗体独立方法来评估四种人类淋巴细胞株的N-糖蛋白质组。总共有404个细胞表面N-糖蛋白作为特定细胞类型的标记物与淋巴细胞性恶性肿瘤有关，包括82个以前在细胞表面蛋白图谱中没有描述的B或T细胞的N-糖蛋白。比较分析、分级聚类技术和无标记定量技术被用来揭示每种细胞类型的最具信息量的蛋白质。毫无疑问，淋巴系恶性肿瘤细胞表面蛋白质组的特征是朝着改进白血病和淋巴瘤的个性化诊断和治疗迈出的第一步。
Lymphocytes are immune cells that are critical for the maintenance of adaptive immunity. Differentiation of lymphoid progenitors yields B-, T-, and NK-cell subtypes that individually correlate with specific forms of leukemia or lymphoma. Therefore, it is imperative a precise method of cell categorization is utilized to detect differences in distinct disease states present in patients. One viable means of classification involves evaluation of the cell surface proteome of lymphoid malignancies. Specifically, this manuscript details the use of an antibody independent approach known as Cell Surface Capture Technology, to assess N-glycoproteome of four human lymphocyte cell lines. Altogether, 404 cell surface N-glycoproteins as markers for specific cell types involved in lymphocytic malignancies, including 82 N-glycoproteins that had not been previously been described for B- or T-cells within the Cell Surface Protein Atlas. Comparative analysis, hierarchical clustering techniques, and label free quantitation was used to reveal proteins most informative for each cell type. Undoubtedly, the characterization of the cell surface proteome of lymphoid malignancies is a first step towards improving personalized diagnosis and treatment of leukemia and lymphoma.
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Wollscheid B
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