Cholinergic control of bone development and beyond.
Cholinergic control of bone development and beyond.
复制标题
胆碱能控制骨骼发育及其他
DOI：
10.1016/j.intimp.2020.106405
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发表时间：
2020
影响因子：
5.6
通讯作者：
Paul G.
中科院分区：
文献类型：
--
作者：
Spieker;Janine;Frieß;Johannes L;Sperling;Thangaraj;Gopenath;Vogel- Höpker;Paul G.
有充分的证据表明，胆碱能行动影响骨骼的健康状况，包括man. Nicotine的脊椎动物吸烟，但也暴露于农药或医疗药物点的意义，胆碱能作用对骨骼的状态，在这里审查的介绍。然后，我们概述了软骨内骨化的过程，并回顾各自的胆碱能作用。在结果部分，我们简要总结了我们在鸡和小鼠体内和体外骨发育的研究[1，2]，包括：（i）胆碱能成分的表达（ii）在产前ChE敲除小鼠中骨骼发生期间缺陷的表征，（iii）用浸泡在胆碱能组分中的珠粒并植入鸡肢芽的功能丧失实验，最后（iv）我们使用了模拟软骨和骨形成的体外间充质3D微质量模型，该模型也揭示了胆碱能、辐射和炎症机制之间的复杂串扰[3]。在讨论中，我们通过考虑以下因素来评估胆碱酯酶在骨形成过程中的非胆碱能作用：（i）胆碱酯酶如何在粘附机制中发挥作用;（ii）胆碱酯酶是否以及如何与碱性磷酸酶（ALP）和/或ECM组分形成骨调节复合物，从而调节细胞分裂，迁移和粘附。我们得出结论，骨骼发育中的胆碱能作用主要由经典的胆碱能，但非神经周期（例如，乙酰胆碱）;此外，这两种胆碱酯酶可以发挥不同的乙酰胆碱独立的作用。考虑到其巨大的医学影响，这些结果提出了新的研究方向，值得追求。
There is ample evidence that cholinergic actions affect the health status of bones in vertebrates including man. Nicotine smoking, but also exposure to pesticides or medical drugs point to the significance of cholinergic effects on bone status, as reviewed here in Introduction. Then, we outline processes of endochondral ossification, and review respective cholinergic actions. In Results, we briefly summarize ourin vivoandin vitrostudies on bone development of chick and mouse [1,2], including (i) expressions of cholinergic components (AChE, BChE, ChAT) in chick embryo, (ii) characterisation of defects during skeletogenesis in prenatal ChE knockout mice, (iii) loss-of-function experiments with beads soaked in cholinergic components and implanted into chicken limb buds, and finally (iv) we use anin vitromesenchymal 3D-micromass model that mimics cartilage and bone formation, which also had revealed complex crosstalks between cholinergic, radiation and inflammatory mechanisms [3]. In Discussion, we evaluate non-cholinergic actions of cholinesterases during bone formation by considering: (i) how cholinesterases could function in adhesive mechanisms; (ii) whether and how cholinesterases can form bone-regulatory complexes with alkaline phosphatase (ALP) and/or ECM components, which could regulate cell division, migration and adhesion. We conclude that cholinergic actions in bone development are driven mainly by classic cholinergic, but non-neural cycles (e.g., by acetylcholine); in addition, both cholinesterases can exert distinct ACh-independent roles. Considering their tremendous medical impact, these results bring forward novel research directions that deserve to be pursued.
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影响因子：
3.7
作者：
Spieker J;Ackermann A;Salfelder A;Vogel-Höpker A;Layer PG
通讯作者：
Layer PG
影响因子：
3.5
作者：
Paraoanu, LE;Layer, PG
通讯作者：
Layer, PG
影响因子：
4.8
作者：
Matsubara, Takuma;Kida, Kumiko;Yoneda, Toshiyuki
通讯作者：
Yoneda, Toshiyuki
鸡视网膜和顶盖“非分层”和“分层”细胞聚集体中的胆碱酯酶和细胞增殖
影响因子：
3.6
作者：
G. Vollmer;P. Layer
通讯作者：
P. Layer
尼古丁对骨愈合的影响。
DOI：
--
发表时间：
1999
期刊：
Journal of Biomedical Materials Research
影响因子：
--
作者：
Jeffrey O. Hollinger;John M. Schmitt;Kun Hwang;Peiman Soleymani;D. Buck
通讯作者：
D. Buck