Molecular Cloning of the B4GALNT2 Gene and Its Single Nucleotide Polymorphisms Association with Litter Size in Small Tail Han Sheep.
Molecular Cloning of the B4GALNT2 Gene and Its Single Nucleotide Polymorphisms Association with Litter Size in Small Tail Han Sheep.
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DOI：
10.3390/ani8100160
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发表时间：
2018-09-20
期刊：
影响因子：
--
通讯作者：
Chu M
中科院分区：
文献类型：
--
作者：
Guo X;Wang X;Liang B;Di R;Liu Q;Hu W;He X;Zhang J;Zhang X;Chu M
在法国Lacaune绵羊中，B4 GALNT 2（β-1，4-N-乙酰氨基半乳糖转移酶2）基因被认为是FecL（突变）的潜在基因，该基因调节绵羊排卵率。与FecL突变相关的3个特异性突变位点在11个绵羊品种中均未发现。而B4 GALNT 2基因外显子g.36946470C > T和g.36933082C > T突变对小尾寒羊第一胎产羔数有显著影响（p < 0.05）。B4 GALNT 2主要在绵羊卵巢中表达，在绵羊生殖过程中也发挥重要作用。在体外培养的绵羊颗粒细胞中，我们发现B4 GALNT 2在其5′-侧翼区有两个转录起始位点（TSS）。在法国Lacaune绵羊中发现了一个新的繁殖力基因，命名为FecL（突变），它调节排卵率。B4 GALNT 2（β-1，4-N-乙酰氨基半乳糖转移酶2）基因被认为是潜在的FecL突变基因。本研究旨在探讨FecL突变对其他绵羊品种的影响，以及B4 GALNT 2基因的分子结构和表达谱特征。利用桑格测序技术，在11个被测绵羊品种中，高、低繁殖力品种均未发现与FecL突变连锁的3个特异性突变位点。而B4 GALNT 2外显子g.36946470C > T和g.36933082C > T两个突变对小尾寒羊第一胎产羔数有显著影响（p < 0.05）。通过RACE（Rapid amplification of cDNA ends）方法，在体外培养的绵羊颗粒细胞中发现了B4 GALNT 2基因5′侧翼区的两个转录起始位点（TSS）。STH绵羊和滩羊之间除肾脏和输卵管外，其他部位的表达量均无显著差异。B4 GALNT 2基因作为FecL的候选基因，可能与STH绵羊产羔数的差异有关。B4 GALNT 2主要在绵羊卵巢中表达，这也表明B4 GALNT 2在绵羊生殖中起着重要作用。
In French Lacaune sheep, the B4GALNT2 (beta-1, 4-N-acetyl-galactosaminyl transferase 2) gene was considered as the potential gene for a FecL (mutation), which regulates the ovine ovulation rate. Three specific mutation sites linked with the FecL mutation have not been previously found in 11 sheep breeds. However, two mutations of g.36946470C > T and g.36933082C > T in the exon of B4GALNT2 were found to have had a significant effect on the litter size in the first parity for Small Tail Han (STH) Sheep (p < 0.05). B4GALNT2, which is mainly expressed in ovine ovary, also plays an important role in sheep reproduction. Furthermore, we discovered two transcription start sites (TSS) of B4GALNT2 in its 5′-flanking region in ovine granule cells in vitro. A new fecundity gene named the FecL (mutation), which regulates the ovulation rate, was discovered in French Lacaune sheep. The B4GALNT2 (beta-1, 4-N-acetyl-galactosaminyl transferase 2) gene was considered as the potential FecL mutation gene. This study explores whether the effect of the FecL mutation exists in other sheep breeds, and the features of the B4GALNT2 gene in terms of the molecular structure and its expression profile. Using Sanger sequencing, we found that high and low fecundity breeds from among 11 measured sheep breeds all had no variation in the three specific mutation sites, which were linked with the FecL mutation. However, two mutations of g.36946470C > T and g.36933082C > T in the exon of B4GALNT2 had a significant effect on litter size in the first parity for Small Tail Han (STH) Sheep (p < 0.05). Two transcription start sites (TSS) of B4GALNT2 in its 5′-flanking region were discovered in ovine granule cells in vitro, through the RACE (Rapid amplification of cDNA ends) method. Except for in the kidney and oviduct, no significant difference in expression levels had been found between STH sheep and Tan sheep breeds. The B4GALNT2 gene, as a candidate for FecL, may have a relationship with the differences in litter size in STH sheep. B4GALNT2 is mainly expressed in the ovine ovary, which also suggests that B4GALNT2 plays an important role in sheep reproduction.
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影响因子：
4.1
作者：
Bodin, L;SanCristobal, M;Elsen, JM
通讯作者：
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4.1
作者：
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