RZZ-Spindly and CENP-E form an integrated platform to recruit dynein to the kinetochore corona.
RZZ-Spindly and CENP-E form an integrated platform to recruit dynein to the kinetochore corona.
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DOI：
10.15252/embj.2023114838
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发表时间：
2023-12-11
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Chromosome biorientation on the mitotic spindle is prerequisite to errorless genome inheritance. CENP‐E (kinesin‐7) and dynein–dynactin (DD), microtubule motors with opposite polarity, promote biorientation from the kinetochore corona, a polymeric structure whose assembly requires MPS1 kinase. The corona's building block consists of ROD, Zwilch, ZW10, and the DD adaptor Spindly (RZZS). How CENP‐E and DD are scaffolded and mutually coordinated in the corona remains unclear. Here, we show that when corona assembly is prevented through MPS1 inhibition, CENP‐E is absolutely required to retain RZZS at kinetochores. An RZZS phosphomimetic mutant bypasses this requirement, demonstrating the existence of a second receptor for polymeric RZZS. With active MPS1, CENP‐E is dispensable for corona expansion, but strictly required for physiological kinetochore accumulation of DD. Thus, we identify the corona as an integrated scaffold where CENP‐E kinesin controls DD kinetochore loading for coordinated bidirectional transport of chromosome cargo. The kinesin‐7 CENP‐E is essential for the kinetochore recruitment of RZZS complex building blocks and fibrous corona expansion when RZZS polymerization is blocked by MPS1 kinase inhibition.
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影响因子：
3.3
作者：
Barisic M;Sohm B;Mikolcevic P;Wandke C;Rauch V;Ringer T;Hess M;Bonn G;Geley S
通讯作者：
Geley S
影响因子：
16.6
作者：
Chakraborty, Manas;Tarasovetc, Ekaterina, V;Grishchuk, Ekaterina L.
通讯作者：
Grishchuk, Ekaterina L.
影响因子：
7.7
作者：
Alex, Amal;Piano, Valentina;Maffini, Stefano
通讯作者：
Maffini, Stefano
DOI：
10.1083/jcb.201311113
发表时间：
2014-03-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ballister ER;Riegman M;Lampson MA
通讯作者：
Lampson MA
DOI：
10.1083/jcb.200805185
发表时间：
2008-10-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yamamoto TG;Watanabe S;Essex A;Kitagawa R
通讯作者：
Kitagawa R