SPDL-1 functions as a kinetochore receptor for MDF-1 in Caenorhabditis elegans.
SPDL-1 functions as a kinetochore receptor for MDF-1 in Caenorhabditis elegans.
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DOI：
10.1083/jcb.200805185
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发表时间：
2008-10-20
期刊：
影响因子：
--
通讯作者：
Kitagawa R
中科院分区：
文献类型：
--
作者：
Yamamoto TG;Watanabe S;Essex A;Kitagawa R
The spindle assembly checkpoint (SAC) ensures faithful chromosome segregation by delaying anaphase onset until all sister kinetochores are attached to bipolar spindles. An RNA interference screen for synthetic genetic interactors with a conserved SAC gene, san-1/MAD3, identified spdl-1, a Caenorhabditis elegans homologue of Spindly. SPDL-1 protein localizes to the kinetochore from prometaphase to metaphase, and this depends on KNL-1, a highly conserved kinetochore protein, and CZW-1/ZW10, a component of the ROD–ZW10–ZWILCH complex. In two-cell–stage embryos harboring abnormal monopolar spindles, SPDL-1 is required to induce the SAC-dependent mitotic delay and localizes the SAC protein MDF-1/MAD1 to the kinetochore facing away from the spindle pole. In addition, SPDL-1 coimmunoprecipitates with MDF-1/MAD1 in vivo. These results suggest that SPDL-1 functions in a kinetochore receptor of MDF-1/MAD1 to induce SAC function.
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DOI：
10.1083/jcb.153.6.1209
发表时间：
2001-06-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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