Generating human intestinal tissue from pluripotent stem cells in vitro.
Generating human intestinal tissue from pluripotent stem cells in vitro.
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DOI：
10.1038/nprot.2011.410
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发表时间：
2011-11-10
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
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作者：
我们描述了一种从人多能干细胞体外生成三维人肠组织（称为类器官）的方案。为了产生肠类器官，首先通过用激活素A处理细胞3天将多能干细胞分化成F0 XA 2 +/S 0X 17+内胚层。在内胚层诱导后，使用FGF 4和WNT 3a将多能干细胞图案化为CDX 2+中肠/后肠组织。在该图案化步骤期间，三维中/后肠球状体从附着于组织培养皿的单层上皮出芽。将三维球状体在基质胶中与促肠生长因子一起沿着进一步培养，并在1-3个月内增殖和扩增以产生肠组织，完整的肠组织具有由所有主要肠细胞类型组成的肠间充质和上皮。到目前为止，这是唯一的方法，有效地直接分化的人多能干细胞成三维人体肠道组织在体外。
We describe a protocol to generate 3-dimensional human intestinal tissue (called organoids) in vitro from human pluripotent stem cells. To generate intestinal organoids, pluripotent stem cells are first differentiated into FOXA2+/SOX17+ endoderm by treating the cells with ActivinA for 3 days. Following endoderm induction, the pluripotent stem cells are patterned into CDX2+ mid/hindgut tissue using FGF4 and WNT3a. During this patterning step, 3-dimensional mid/hindgut spheroids bud from the monolayer epithelium attached to the tissue culture dish. The 3-dimensional spheroids are further cultured in matrigel along with pro-intestinal growth factors, and proliferate and expand over 1–3 months to give rise to intestinal tissue, complete with intestinal mesenchyme and epithelium consisting of all of the major intestinal cell types. To date, this is the only method to efficiently direct differentiation of human pluripotent stem cells into 3-dimensional human intestinal tissue in vitro.
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DOI：
10.1002/dvdy.22540
发表时间：
2011-03
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Spence JR;Lauf R;Shroyer NF
通讯作者：
Shroyer NF
影响因子：
5.2
作者：
Matsuura, Rie;Kogo, Hiroshi;Torihashi, Shigeko
通讯作者：
Torihashi, Shigeko
影响因子：
5.2
作者：
Yamada, T;Yoshikawa, M;Tsunoda, Y
通讯作者：
Tsunoda, Y
影响因子：
64.8
作者：
Spence, Jason R.;Mayhew, Christopher N.;Rankin, Scott A.;Kuhar, Matthew F.;Vallance, Jefferson E.;Tolle, Kathryn;Hoskins, Elizabeth E.;Kalinichenko, Vladimir V.;Wells, Susanne I.;Zorn, Aaron M.;Shroyer, Noah F.;Wells, James M.
通讯作者：
Wells, James M.
影响因子：
4
作者：
Konuma, Noriyoshi;Wakabayashi, Kumiko;Mugishima, Hideo
通讯作者：
Mugishima, Hideo