Long-term exposure to dietary sources of genistein induces estrogen-independence in the human breast cancer (MCF-7) xenograft model.
Long-term exposure to dietary sources of genistein induces estrogen-independence in the human breast cancer (MCF-7) xenograft model.
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DOI：
10.1002/mnfr.201300780
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发表时间：
2015-03
影响因子：
5.2
通讯作者：
Helferich, William G.
中科院分区：
文献类型：
--
作者：
Andrade, Juan E.;Ju, Young H.;Baker, Chandra;Doerge, Daniel R.;Helferich, William G.
在裸鼠异种移植模型中研究了暴露于相关膳食水平的染料木黄酮（GEN）对雌激素受体阳性（ER+）人乳腺癌（MCF-7）进展的长期影响。进行饲养研究以确定饮食对MCF-7肿瘤生长的雌激素效应：1）植入17β-雌二醇（E2）治疗/停药（19周）和停药（6周）; 2）在治疗/停药期间分别饲喂GEN 500和750 ppm，持续23/10周和15/9周;和，3）含有GEN 180 ppm的膳食大豆分离蛋白（SPI），治疗/停药31/9周。MCF-7肿瘤在E2植入的存在下快速生长，并且在E2撤回后突然完全消退。在不同的速率下，单独的饮食GEN（500和750 ppm）和含GEN（180 ppm）的SPI刺激MCF-7肿瘤生长。去除刺激饮食后，由750 ppm GEN诱导的肿瘤完全消退，但500 ppm GEN或SPI则没有。与MCF-7和E2对照组相比，GEN和SPI诱导的非消退（GINR）肿瘤中表皮生长因子受体2（HER 2）的蛋白表达更高。长期摄入低剂量GEN（≤500 ppm）可促进MCF-7肿瘤生长，并导致GINR肿瘤具有更强的侵袭性和晚期生长表型。
The long-term effect of exposure to relevant dietary levels of genistein (GEN) on estrogen receptor-positive (ER+) human breast cancer (MCF-7) progression after GEN withdrawal in athymic mice xenograft model was studied. Feeding studies were conducted to determine the estrogenic effect of diets on MCF-7 tumor growth: 1) implantation (19 weeks) and withdrawal (6 weeks) of 17β-estradiol (E2); 2) dietary GEN 500 and 750 ppm during treatment/withdrawal for 23/10 and 15/9 weeks, respectively; and, 3) dietary soy protein isolate (SPI) containing GEN 180 ppm for 31/9 weeks of treatment/withdrawal. MCF-7 tumors grew fast in the presence of E2 implantation and abruptly regressed completely after E2 withdrawal. At different rates, dietary GEN alone (500 and 750 ppm) and GEN (180 ppm)-containing SPI stimulated MCF-7 tumor growth. After removal of the stimulus diet, tumors induced by 750 ppm GEN, but not 500 ppm GEN or SPI, regressed completely. The protein expression of epidermal growth factor receptor 2 (HER2) was higher in the GEN- and SPI-induced non-regressing (GINR) tumors compared to MCF-7 and E2 controls. Long-term consumption of low GEN doses (≤500 ppm) promotes MCF-7 tumor growth and results in GINR tumors with more aggressive and advanced growth phenotypes.
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影响因子：
5.8
作者：
Helferich, W. G.;Andrade, J. E.;Hoagland, M. S.
通讯作者：
Hoagland, M. S.
影响因子：
6.1
作者：
Andrade JE;Twaddle NC;Helferich WG;Doerge DR
通讯作者：
Doerge DR
影响因子：
4.8
作者：
Kuiper, GGJM;Lemmen, JG;Gustafsson, JÄ
通讯作者：
Gustafsson, JÄ
影响因子：
45.3
作者：
CLARK, GM;OSBORNE, CK;MCGUIRE, WL
通讯作者：
MCGUIRE, WL
影响因子：
4.7
作者：
Ju, Young H.;Doerge, Daniel R.;Helferich, William G.
通讯作者：
Helferich, William G.