Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue.
Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue.
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DOI：
10.1038/s41598-018-31178-y
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发表时间：
2018-08-24
影响因子：
4.6
通讯作者：
Michael R
中科院分区：
文献类型：
--
作者：
D'Antin JC;Barraquer RI;Tresserra F;Michael R
为了确定白内障手术后后囊膜混浊是否可以通过应用过氧化氢（H2 O2）或蒸馏水（H2Od）来延迟或抑制，我们从25个人类供体眼球中提取了透镜囊。将样品用30 mM H2 O2或H2Od处理5 min或用作对照，并培养一个月，在此期间拍摄暗场和倾斜照明照片。这些用于观察和量化后囊上细胞生长和汇合的时间。培养后，对组织切片进行H&E、α-SMA、Ki-67和波形蛋白染色并进行评价。我们防止细胞生长在50%的H2Od和58%的H2 O2处理的样品。与培养的对照相比，两种处理对细胞生长的总体预防是显著的，但它们之间没有显著差异。在没有阻止细胞生长的情况下，两种治疗都显著延迟细胞生长。直到第28天，显示生长的任一类型的处理样品都没有达到完全汇合。所有培养的对照在处理样品前达到完全汇合（中位数=第11.5天）。此外，在组织学上，培养对照和处理样品之间存在明显的形态学差异。
In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (H2O2) or distilled water (H2Od),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM H2O2 or H2Od or used as controls, and cultured for one month, during which dark field and tilt illumination photos were taken. These were used to observe and quantify, time until cellular growth and confluence on the posterior capsule. After culture, histological sections were stained for H&E, α-SMA, Ki-67 and vimentin and evaluated. We prevented cellular growth in 50% of H2Od and 58% H2O2 of treated samples. The overall prevention of cell growth compared to cultured controls was significant for both treatments while there was no significant difference between them. In the cases where cellular growth was not prevented, both treatments significantly delay cellular growth. Until day 28 none of the treated samples of either type that had shown growth reached total confluence. All cultured controls reached total confluence before treated samples (median = day 11.5). Also, histologically, there was a clear morphological difference between cultured controls and treated samples.
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影响因子：
4.6
作者：
Eldred JA;McDonald M;Wilkes HS;Spalton DJ;Wormstone IM
通讯作者：
Wormstone IM
影响因子：
2.8
作者：
Meacock, WR;Spalton, DJ;Sanguinetti, G
通讯作者：
Sanguinetti, G
影响因子：
2.8
作者：
Davison, JA
通讯作者：
Davison, JA
影响因子：
2.8
作者：
Pepose, Jay S.;Hayashida, Jon;Feinerman, Gregg
通讯作者：
Feinerman, Gregg
DOI：
10.1016/0304-4165(77)90284-7
发表时间：
1977-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BHUYAN, KC;BHUYAN, DK
通讯作者：
BHUYAN, DK