Next-generation sequencing from bulked segregant analysis identifies a dwarfism gene in watermelon.
Next-generation sequencing from bulked segregant analysis identifies a dwarfism gene in watermelon.
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DOI：
10.1038/s41598-018-21293-1
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发表时间：
2018-02-13
影响因子：
4.6
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Dong W;Wu D;Li G;Wu D;Wang Z
矮生性是西瓜育种中最有价值的性状之一，主要是因为其对产量的贡献以及培养和收获较小植株所需的劳动力减少。然而，其潜在的遗传机制尚不清楚。本研究应用新一代测序技术对西瓜植株进行分析，筛选出一个矮秆基因候选基因。我们完成了一个全基因组重测序的两个DNA块（矮池和藤池）产生的植物在F2群体。一个全基因组的单核苷酸多态性分析，导致在检测的基因组区域窝藏候选侏儒症基因Cla 010726。预测编码的蛋白质为赤霉素20-氧化酶样蛋白，这是其他作物中众所周知的“绿色革命”蛋白。实时荧光定量PCR检测结果表明，Cla 010726基因在矮秆植株中的表达量显著低于正常大小植株。单核苷酸多态性（SNP）分析结果表明，dsh F2的Cla 010726基因启动子区存在2个SNP位点。本研究结果为Cla 010726可能是一个矮秆基因提供了初步证据。
Dwarfism is one of the most valuable traits in watermelon breeding mainly because of its contribution to yield as well as the decreased labor required to cultivate and harvest smaller plants. However, the underlying genetic mechanism is unknown. In this study, a candidate dwarfism gene was identified by applying next-generation sequencing technology to analyze watermelon plants. We completed a whole-genome re-sequencing of two DNA bulks (dwarf pool and vine pool) generated from plants in an F2 population. A genome-wide analysis of single nucleotide polymorphisms resulted in the detection of a genomic region harboring the candidate dwarfism gene Cla010726. The encoded protein was predicted to be a gibberellin 20-oxidase-like protein, which is a well-known “green revolution” protein in other crops. A quantitative real-time PCR investigation revealed that the Cla010726 expression level was significantly lower in the dwarf plants than in the normal-sized plants. The SNP analysis resulted in two SNP locating in the Cla010726 gene promoter of dsh F2 individuals. The results presented herein provide preliminary evidence that Cla010726 is a possible dwarfism gene.
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影响因子：
4.3
作者：
Huang, HX;Zhang, XQ;Li, X
通讯作者：
Li, X
影响因子：
3.1
作者：
Hwang, Jihyun;Oh, Juyoul;Park, Younghoon
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影响因子：
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作者：
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通讯作者：
Chong, Kang
DOI：
10.1073/pnas.91.18.8552
发表时间：
1994-08-30
影响因子：
11.1
作者：
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通讯作者：
GRAEBE, JE
影响因子：
4.3
作者：
Holst, Kerstin;Schmuelling, Thomas;Werner, Tomas
通讯作者：
Werner, Tomas