The Transcriptional Response of Aedes aegypti with Variable Extrinsic Incubation Periods for Dengue Virus.
The Transcriptional Response of Aedes aegypti with Variable Extrinsic Incubation Periods for Dengue Virus.
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DOI：
10.1093/gbe/evy230
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发表时间：
2018-12-01
影响因子：
3.3
通讯作者：
Chenoweth SF
中科院分区：
文献类型：
--
作者：
Koh C;Allen SL;Herbert RI;McGraw EA;Chenoweth SF
登革热是全球最流行的虫媒病毒疾病。登革热病毒主要由埃及伊蚊传播。蚊子作为媒介的效率的一个衡量标准是外在潜伏期（EIP），这是摄入病毒血症血液和唾液中病毒粒子出现之间的时间。病毒感染中肠并进入唾液的时间越长，蚊子在其一生中传播病原体的机会就越少。我们以前已经表明，登革病毒的EIP是高度遗传的，它与载体寿命呈负相关。在这里，我们研究了蚊子的转录谱，不同的EIP表型，并确定了与短或长EIP相关的途径。我们发现，短EIP的蚊子具有较低的主动免疫应答，但具有较高水平的蛋白质翻译和钙离子稳态，并且具有较长EIP的蚊子可能具有较慢的代谢。这些发现表明钙离子分布、核糖体生物发生和代谢之间存在复杂的相互作用，并揭示了可能被修改以减缓病毒进展速度并因此限制终身传播能力的潜在途径。
Dengue fever is the most prevalent arboviral disease globally. Dengue virus is transmitted primarily by the Aedes aegypti mosquito. One measure of the mosquito’s efficiency as a vector is the extrinsic incubation period (EIP), which is the time between the ingestion of viremic blood and the emergence of virions in the saliva. The longer it takes virus to infect the midgut and traverse to the saliva, the fewer opportunities the mosquito will have to transmit the pathogen over its lifetime. We have shown previously that EIP for dengue virus is highly heritable and that it is negatively correlated with vector lifespan. Here, we examined the transcriptional profiles for mosquitoes that varied in their EIP phenotype and identified pathways associated with either short or long EIP. We found that mosquitoes with short EIP have less active immune responses but higher levels of protein translation and calcium ion homeostasis and that mosquitoes with longer EIP may have slower metabolism. These findings indicate a complex interplay between calcium ion distribution, ribosome biogenesis, and metabolism and reveal potential pathways that could be modified to slow the rate of viral progression and hence limit lifetime transmission capability.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
4.8
作者：
Hammadi, Mehdi;Oulidi, Agathe;Van Coppenolle, Fabien
通讯作者：
Van Coppenolle, Fabien
影响因子：
3.7
作者：
Frentiu FD;Robinson J;Young PR;McGraw EA;O'Neill SL
通讯作者：
O'Neill SL
影响因子：
9.2
作者：
Copeland, Jeffrey M.;Cho, Jaehyoung;Walker, David W.
通讯作者：
Walker, David W.
影响因子：
3.8
作者：
Adams, Ben;Boots, Michael
通讯作者：
Boots, Michael