Superfast excitation-contraction coupling in adult zebrafish skeletal muscle fibers.
Superfast excitation-contraction coupling in adult zebrafish skeletal muscle fibers.
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DOI：
10.1085/jgp.202213158
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发表时间：
2022-09-05
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影响因子：
--
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斑马鱼表现出一种逃跑反应，这种反应是由游动肌肉的超高速收缩提供动力的。Idoux等人表明，收缩前的步骤，从动作电位放电到细胞内Ca2+的增加，显示出在脊椎动物运动肌肉中测量到的最快动力学。斑马鱼已经成为探索人类骨骼肌疾病病理生理学的一个非常相关的动物模型。这种脊椎动物模型显示出一种以高频游泳活动为特征的惊吓反应，这种活动是由以极高频率兴奋的快速骨骼肌纤维收缩提供动力的，这对逃脱捕食者和捕获猎物至关重要。这种强烈的肌肉表现需要非常快的收缩机械特性，也需要兴奋-收缩耦合，通过沿肌膜扩散的动作电位诱导二氢吡啶受体结构的变化，导致膜内电荷运动，进而触发肌浆网Ca2+的释放。然而，到目前为止，由脊椎动物肌肉纤维引起的最快的Ca2+瞬变已被描述为用于产生声音的肌肉，如蟾蜍鱼的鱼鳔，而不是用于运动的肌肉。通过在电压控制下对成年斑马鱼和小鼠分离的快骨骼肌纤维进行细胞内Ca2+测量，我们证明了鱼快肌纤维表现出超快的动作电位动力学、膜内电荷运动和动作电位诱发的Ca2+瞬态。允许融合和融合持续的Ca2+瞬态，其激发频率远高于小鼠快速骨骼肌纤维，与产生声音的肌肉中记录的频率相当。本研究首次证明了骨骼肌的兴奋-收缩耦合的超快动力学，从而允许脊椎动物的超快运动行为。
The zebrafish displays an escape response powered by superfast contraction of swimming muscle. Idoux et al. show that steps preceding contraction, from action potential firing to increase in intracellular Ca2+, display the fastest kinetics ever measured in vertebrate locomotor muscles. The zebrafish has emerged as a very relevant animal model for probing the pathophysiology of human skeletal muscle disorders. This vertebrate animal model displays a startle response characterized by high-frequency swimming activity powered by contraction of fast skeletal muscle fibers excited at extremely high frequencies, critical for escaping predators and capturing prey. Such intense muscle performance requires extremely fast properties of the contractile machinery but also of excitation–contraction coupling, the process by which an action potential spreading along the sarcolemma induces a change in configuration of the dihydropyridine receptors, resulting in intramembrane charge movements, which in turn triggers the release of Ca2+ from the sarcoplasmic reticulum. However, thus far, the fastest Ca2+ transients evoked by vertebrate muscle fibers has been described in muscles used to produce sounds, such as those in the toadfish swim bladder, but not in muscles used for locomotion. By performing intracellular Ca2+ measurements under voltage control in isolated fast skeletal muscle fibers from adult zebrafish and mouse, we demonstrate that fish fast muscle fibers display superfast kinetics of action potentials, intramembrane charge movements, and action potential–evoked Ca2+ transient, allowing fusion and fused sustained Ca2+ transients at frequencies of excitation much higher than in mouse fast skeletal muscle fibers and comparable to those recorded in muscles producing sounds. The present study is the first demonstration of superfast kinetics of excitation–contraction coupling in skeletal muscle allowing superfast locomotor behaviors in a vertebrate.
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影响因子：
3.8
作者：
Ferreira Gregorio, Juan;Pequera, German;Brum, Gustavo
通讯作者：
Brum, Gustavo
DOI：
10.1085/jgp.201210773
发表时间：
2012-04
期刊：
The Journal of general physiology
影响因子：
--
作者：
Baylor SM;Hollingworth S
通讯作者：
Hollingworth S
DOI：
10.1111/febs.12412
发表时间：
2013-09
期刊：
The FEBS journal
影响因子：
--
作者：
Gibbs EM;Horstick EJ;Dowling JJ
通讯作者：
Dowling JJ
影响因子：
4
作者：
Schrötter K;Dayal A;Grabner M
通讯作者：
Grabner M
影响因子：
4
作者：
Idoux, Romane;Fuster, Clarisse;Allard, Bruno
通讯作者：
Allard, Bruno