Potential associations between severity of infection and the presence of virulence-associated genes in clinical strains of Staphylococcus aureus.
Potential associations between severity of infection and the presence of virulence-associated genes in clinical strains of Staphylococcus aureus.
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DOI：
10.1371/journal.pone.0018673
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发表时间：
2011-04-26
期刊：
影响因子：
3.7
通讯作者：
Fowler VG Jr
中科院分区：
文献类型：
--
作者：
Gill SR;McIntyre LM;Nelson CL;Remortel B;Rude T;Reller LB;Fowler VG Jr
金黄色葡萄球菌感染的临床范围从无症状的鼻腔携带到骨髓炎、感染性心内膜炎（IE）和死亡。在这项研究中，我们评估了在一组前瞻性特征的沙门氏菌中特定基因的存在之间的潜在关联。金黄色葡萄球菌临床分离株和临床结果。239个S。金黄色葡萄球菌分离株（121株耐甲氧西林的S.金黄色葡萄球菌[MRSA]和118株甲氧西林敏感的S.金黄色葡萄球菌[MSSA]）进行筛选，以鉴定与复杂感染有关的基因。经过多次测试调整后，226个基因与感染严重程度显著相关。在这226个基因中，185个不在SCCmec元件中。在185个非SCCmec基因中，171个在复杂感染组中较不常见，14个较常见。在SCCmec元件的41个基因中，37个在复杂组中较常见，4个较不常见。在2014年评估的序列中，共有51个序列（14个非SCCmec和37个SCCmec）被鉴定为目标基因。在复杂感染中不常见的171个基因中，有152个基因的功能未知，可能有助于减弱毒力。在复杂感染中更常见的14种非SCCmec基因包括噬菌体编码的基因，例如在组织粘附或生物膜形成中具有潜在作用的调节因子和自溶素。
The clinical spectrum of Staphylococcus aureus infection ranges from asymptomatic nasal carriage to osteomyelitis, infective endocarditis (IE) and death. In this study, we evaluate potential association between the presence of specific genes in a collection of prospectively characterized S. aureus clinical isolates and clinical outcome. Two hundred thirty-nine S. aureus isolates (121 methicillin-resistant S. aureus [MRSA] and 118 methicillin-susceptible S. aureus [MSSA]) were screened by array comparative genomic hybridization (aCGH) to identify genes implicated in complicated infections. After adjustment for multiple tests, 226 genes were significantly associated with severity of infection. Of these 226 genes, 185 were not in the SCCmec element. Within the 185 non-SCCmec genes, 171 were less common and 14 more common in the complicated infection group. Among the 41 genes in the SCCmec element, 37 were more common and 4 were less common in the complicated group. A total of 51 of the 2014 sequences evaluated, 14 non-SCCmec and 37 SCCmec, were identified as genes of interest. Of the 171 genes less common in complicated infections, 152 are of unknown function and may contribute to attenuation of virulence. The 14 non-SCCmec genes more common in complicated infections include bacteriophage-encoded genes such as regulatory factors and autolysins with potential roles in tissue adhesion or biofilm formation.
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影响因子：
6.4
作者：
Fowler, Vance G., Jr.;Nelson, Charlotte L.;Gill, Steven R.
通讯作者：
Gill, Steven R.
影响因子：
3.2
作者：
Lindsay, JA;Moore, CE;Hinds, J
通讯作者：
Hinds, J
影响因子：
3.3
作者：
Benjamini, Y;Yekutieli, D
通讯作者：
Yekutieli, D
影响因子：
3.6
作者：
Goerke, Christiane;Wirtz, Christiane;Wolz, Christiane
通讯作者：
Wolz, Christiane
影响因子：
2.6
作者：
Bae, T;Schneewind, O
通讯作者：
Schneewind, O