OXI1 protein kinase is required for plant immunity against Pseudomonas syringae in Arabidopsis.
OXI1 protein kinase is required for plant immunity against Pseudomonas syringae in Arabidopsis.
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DOI：
10.1093/jxb/erp219
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发表时间：
2009
影响因子：
6.9
通讯作者：
Denby KJ
中科院分区：
文献类型：
--
作者：
Petersen LN;Ingle RA;Knight MR;Denby KJ
氧化胁迫诱导拟南芥氧化信号诱导1（OXI 1）丝氨酸/苏氨酸蛋白激酶基因（At 3g 25250）表达。该激酶是所需的根毛发育和基础防御卵菌病原体Hyaloperonospora寄生虫，两个独立的H2 O2介导的过程。在这项研究中，OXI 1在发病过程中的作用进一步表征。与野生型相比，OXI 1突变体对活体营养型细菌病原体假单胞菌的强毒和无毒菌株都更敏感，这表明OXI 1积极调节由病原体相关分子模式识别触发的基础抗性以及效应子触发的免疫力。OXI 1的表达水平似乎是至关重要的安装一个适当的防御反应，因为OXI 1过表达线也显示增加的易感性活体营养型病原体。P. erythingae感染后OXI 1的诱导在空间和时间上与氧化爆发相关。此外，在atrbohD突变体中和施用DPI（NADPH氧化酶的抑制剂）后，诱导减少，表明通过NADPH氧化酶产生的活性氧在该植物-病原体相互作用期间驱动OXI 1表达。
Expression of the Arabidopsis Oxidative Signal-Inducible1 (OXI1) serine/threonine protein kinase gene (At3g25250) is induced by oxidative stress. The kinase is required for root hair development and basal defence against the oomycete pathogen Hyaloperonospora parasitica, two separate H2O2-mediated processes. In this study, the role of OXI1 during pathogenesis was characterized further. Null oxi1 mutants are more susceptible to both virulent and avirulent strains of the biotrophic bacterial pathogen Pseudomonas syringae compared with the wild type, indicating that OXI1 positively regulates both basal resistance triggered by the recognition of pathogen-associated molecular patterns, as well as effector-triggered immunity. The level of OXI1 expression appears to be critical in mounting an appropriate defence response since OXI1 overexpressor lines also display increased susceptibility to biotrophic pathogens. The induction of OXI1 after P. syringae infection spatially and temporally correlates with the oxidative burst. Furthermore, induction is reduced in atrbohD mutants and after application of DPI (an inhibitor of NADPH oxidases) suggesting that reactive oxygen species produced through NADPH oxidases drives OXI1 expression during this plant–pathogen interaction.
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