Opiate dependence induces cell type-specific plasticity of intrinsic membrane properties in the rat juxtacapsular bed nucleus of stria terminalis (jcBNST).
Opiate dependence induces cell type-specific plasticity of intrinsic membrane properties in the rat juxtacapsular bed nucleus of stria terminalis (jcBNST).
复制标题
DOI：
10.1007/s00213-017-4732-4
复制
发表时间：
2017-12
影响因子：
3.4
通讯作者：
Sanna PP
中科院分区：
文献类型：
--
作者：
Francesconi W;Szücs A;Berton F;Koob GF;Vendruscolo LF;Sanna PP
滥用药物可以通过改变突触功效和/或神经元的内在膜特性来改变回路动力学。终纹床核（jcBNST）的前囊亚部具有独特的连接性，使其能够整合皮质和杏仁核的输入，并向杏仁核的中央核（CeA）等区域提供前馈抑制。在这项研究中，我们研究了在大鼠jcBNST神经元的突触和内在特性的变化，在长期戒断吗啡依赖使用传统的电生理方法和动态钳技术相结合。阿片类药物依赖的历史诱导一种形式的细胞类型的特定的可塑性，其特征在于减少内向整流与更多的去极化静息膜电位和膜电阻增加。这种细胞类型也表现出较低的基强度时，刺激直流（DC）脉冲，以及在模拟突触轰击与动态钳放电阈值降低。吗啡依赖也减少兴奋性突触后电位放大，这表明持续性钠电流（INaP）的下调。这些研究结果表明，吗啡依赖的历史导致持续的细胞类型特异性可塑性的被动膜特性的jcBNST神经元群体，导致整体增加的兴奋性，这样的神经元。通过改变扩展杏仁核电路的活动，它们嵌入，jcBNST神经元的整合特性的变化可能有助于与药物依赖和戒断相关的情绪失调。
Drugs of abuse can alter circuit dynamics by modifying synaptic efficacy and/or the intrinsic membrane properties of neurons. The juxtacapsular subdivision of the bed nucleus of stria terminalis (jcBNST) has unique connectivity that positions it to integrate cortical and amygdala inputs and provide feed-forward inhibition to the central nucleus of the amygdala (CeA), among other regions. In this study, we investigated changes in the synaptic and intrinsic properties of neurons in the rat jcBNST during protracted withdrawal from morphine dependence using a combination of conventional electrophysiological methods and the dynamic clamp technique. A history of opiate dependence induced a form of cell-type specific plasticity characterized by reduced inward rectification associated with more depolarized resting membrane potentials and increased membrane resistance. This cell type also showed a lower rheobase when stimulated with direct current (DC) pulses as well as a decreased firing threshold under simulated synaptic bombardment with the dynamic clamp. Morphine dependence also decreased excitatory postsynaptic potential amplification, suggesting the downregulation of the persistent Na+-current (INaP). These findings show that a history of morphine dependence leads to persistent cell type-specific plasticity of the passive membrane properties of a jcBNST neuronal population, leading to an overall increased excitability of such neurons. By altering the activity of extended amygdala circuits where they are embedded, changes in the integration properties of jcBNST neurons may contribute to emotional dysregulation associated with drug dependence and withdrawal.
登录
查看更多内容
亚阈值电压处的瞬态钠电流：EPSP波形激活。
影响因子：
16.2
作者：
Carter BC;Giessel AJ;Sabatini BL;Bean BP
通讯作者：
Bean BP
DOI：
10.1523/jneurosci.5129-08.2009
发表时间：
2009-04-29
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Francesconi W;Berton F;Repunte-Canonigo V;Hagihara K;Thurbon D;Lekic D;Specio SE;Greenwell TN;Chen SA;Rice KC;Richardson HN;O'Dell LE;Zorrilla EP;Morales M;Koob GF;Sanna PP
通讯作者：
Sanna PP
影响因子：
64.8
作者：
Ciocchi, Stephane;Herry, Cyril;Luethi, Andreas
通讯作者：
Luethi, Andreas
影响因子：
3.4
作者：
Buffalari, Deanne M.;See, Ronald E.
通讯作者：
See, Ronald E.
影响因子：
4.7
作者：
Edwards S;Vendruscolo LF;Schlosburg JE;Misra KK;Wee S;Park PE;Schulteis G;Koob GF
通讯作者：
Koob GF