The design and synthesis of circular RNAs.
The design and synthesis of circular RNAs.
复制标题
DOI：
10.1016/j.ymeth.2021.02.020
复制
发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Chen YG
中科院分区：
文献类型：
--
作者：
Obi P;Chen YG
Circular RNAs (circRNAs) are a novel class of RNAs distinguished by their single-stranded, covalently-closed topology. Although initially perceived as rare byproducts of aberrant splicing, circRNAs are now recognized as ubiquitously expressed and functionally significant. These discoveries have led to a growing need for ways to model circRNAs in living cells to advance our understanding of their biogenesis, regulation, and function, and to adopt them as new technologies for application within research and medicine. In this review, we provide an updated summary of approaches used to produce circRNAs in vitro and in vivo, the latter of which has grown considerably in recent years. Given increased interest in the unique functions carried out by individual circRNAs, we further dedicate a section on how to customize synthesized circRNAs for specific biological roles. We focus on the most common applications, including designing circRNAs for protein delivery, to target miRNAs and proteins, to act as fluorescent reporters, and to modulate cellular immunity.
登录
查看更多内容
影响因子：
8.4
作者：
Costello, Alan;Lao, Nga T.;Clynes, Martin
通讯作者：
Clynes, Martin
影响因子：
2.4
作者：
Breuer, Janina;Rossbach, Oliver
通讯作者：
Rossbach, Oliver
影响因子：
4.6
作者：
Abe N;Matsumoto K;Nishihara M;Nakano Y;Shibata A;Maruyama H;Shuto S;Matsuda A;Yoshida M;Ito Y;Abe H
通讯作者：
Abe H
影响因子：
64.8
作者：
Aktas, Tugce;Ilik, Ibrahim Avsar;Akhtar, Asifa
通讯作者：
Akhtar, Asifa
影响因子：
14.9
作者：
Braun, S;Domdey, H;Wiebauer, K
通讯作者：
Wiebauer, K