Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells.
Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells.
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DOI：
10.1083/jcb.200908026
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发表时间：
2010-02-22
期刊：
影响因子：
--
通讯作者：
Panning B
中科院分区：
文献类型：
--
作者：
Fazzio TG;Panning B
凝聚素复合物组分Smc 2和-4的缺失会破坏胚胎干细胞存活所需的表观遗传修饰。在一个RNA干扰屏幕询问调节小鼠胚胎干（ES）细胞染色质结构，我们以前确定了62个基因所需的ES细胞活力。其中Smc 2和Smc-4是两种哺乳动物凝聚素复合物的核心成分。在这项研究中，我们表明，Smc 2和-4，以及另外49的62个基因，敲低（KD）在体细胞的增殖或活力的影响最小。KD后，Smc 2和-4表现出两种表型，这是独特的ES细胞和ES细胞的致死目标之间的独特的：中期阻滞和大大扩大间期核。KD ES细胞核增大是由染色质致密化缺陷引起的，而不是DNA含量的变化。压实的改变与几种表观遗传修饰丰度的改变相吻合。这些数据揭示了凝聚素复合物在ES细胞间期染色质致密化中的独特作用。
Loss of the condensin complex components Smc2 and -4 disrupts epigenetic modifications required for embryonic stem cell survival. In an RNA interference screen interrogating regulators of mouse embryonic stem (ES) cell chromatin structure, we previously identified 62 genes required for ES cell viability. Among these 62 genes were Smc2 and -4, which are core components of the two mammalian condensin complexes. In this study, we show that for Smc2 and -4, as well as an additional 49 of the 62 genes, knockdown (KD) in somatic cells had minimal effects on proliferation or viability. Upon KD, Smc2 and -4 exhibited two phenotypes that were unique to ES cells and unique among the ES cell–lethal targets: metaphase arrest and greatly enlarged interphase nuclei. Nuclear enlargement in condensin KD ES cells was caused by a defect in chromatin compaction rather than changes in DNA content. The altered compaction coincided with alterations in the abundance of several epigenetic modifications. These data reveal a unique role for condensin complexes in interphase chromatin compaction in ES cells.
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影响因子：
9.2
作者：
Kaitna, S;Pasierbek, P;Glotzer, M
通讯作者：
Glotzer, M
影响因子：
64.8
作者：
Gaspar-Maia A;Alajem A;Polesso F;Sridharan R;Mason MJ;Heidersbach A;Ramalho-Santos J;McManus MT;Plath K;Meshorer E;Ramalho-Santos M
通讯作者：
Ramalho-Santos M
影响因子：
7.5
作者：
Belmont, Andrew S.
通讯作者：
Belmont, Andrew S.
影响因子：
10.5
作者：
Hagstrom, KA;Holmes, VF;Meyer, BJ
通讯作者：
Meyer, BJ
影响因子：
9.2
作者：
Aladjem, MI;Spike, BT;Wahl, GM
通讯作者：
Wahl, GM