Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo.

Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo.
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DOI:
10.1038/nsmb.2327
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发表时间:
2012-06-17
影响因子:
16.8
通讯作者:
Fairbrother, William G.
Fairbrother, William G.
中科院分区:
生物学1区
文献类型:
--
作者:
Taggart, Allison J.;DeSimone, Alec M.;Shih, Janice S.;Filloux, Madeleine E.;Fairbrother, William G.

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We present the first large-scale identification of lariats—the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3′ splice-site selection, identified new mechanisms of alternative splicing and mapped the distribution of splicing factors around branchpoints.
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