Intratumoral α-SMA enhances the prognostic potency of CD34 associated with maintenance of microvessel integrity in hepatocellular carcinoma and pancreatic cancer.
Intratumoral α-SMA enhances the prognostic potency of CD34 associated with maintenance of microvessel integrity in hepatocellular carcinoma and pancreatic cancer.
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DOI：
10.1371/journal.pone.0071189
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yu XJ
中科院分区：
文献类型：
--
作者：
Wang WQ;Liu L;Xu HX;Luo GP;Chen T;Wu CT;Xu YF;Xu J;Liu C;Zhang B;Long J;Tang ZY;Yu XJ
微血管密度（MVD）作为血管生成预测因子本身在癌症预后中是无效的。我们评估了瘤内α-平滑肌肌动蛋白（α-SMA）阳性间质细胞密度和MVD在高血管性肝细胞癌（HCC）和低血管性胰腺癌（PC）根治性切除后的预后价值。以305例肝癌患者和57例肝癌患者行根治性切除的肿瘤为样本，构建组织微阵列，免疫染色检测α-SMA和CD34的表达。检查这两种蛋白的预后价值和其他临床病理特征。肝癌中α-SMA低密度和MVD-CD34高密度均与肝内转移和微血管侵袭有关，与肝癌的淋巴结累及和微血管侵袭有关（p<0.05）。虽然单独CD34而不是α-SMA是总生存期和无复发生存期的独立预后因素，但低α-SMA和高CD34的联合是两种肿瘤最差预后的预测因子，并且具有更好的预测患者死亡和早期复发的能力（p<0.01）。α- sma阳性细胞大部分与血管内皮细胞分布重叠，主要分布在血管壁上。微血管完整性差，如高MVD所示，加上血管周围α- sma阳性细胞覆盖率低，与肿瘤切除术后早期复发、不良转移和短生存期有关。这一发现突出了血管质量在肿瘤进展中的重要性，为术后患者预后提供了血管数量的优化补充。
Microvessel density (MVD) as an angiogenesis predictor is inefficient per se in cancer prognosis. We evaluated prognostic values of combining intratumoral alpha-smooth muscle actin (α-SMA)-positive stromal cell density and MVD after curative resection in hypervascular hepatocellular carcinoma (HCC) and hypovascular pancreatic cancer (PC). Tissue microarrays were constructed from tumors of 305 HCC and 57 PC patients who underwent curative resection and analyzed for α-SMA and CD34 expression by immunostaining. Prognostic values of these two proteins and other clinicopathological features were examined. Both low α-SMA density and high MVD-CD34 were associated in HCC with the presence of intrahepatic metastasis and microvascular invasion, and they were related to lymph node involvement and microvascular invasion in PC (p<0.05). Although CD34 alone, but not α-SMA, was an independent prognostic factor for overall survival and recurrence-free survival, the combination of low α-SMA and high CD34 was a predictor of worst prognosis for both types of tumors and had a better power to predict patient death and early recurrence (p<0.01). Furthermore, the results show that distribution of most of the α-SMA-positive cells and vascular endothelial cells overlap, showing major colocalization on vascular walls. Poor microvessel integrity, as indicated by high MVD, together with low perivascular α-SMA-positive cell coverage is associated with early recurrence, unfavorable metastasis, and short survival after tumor resection. This finding highlights the significance of vascular quality in tumor progression, which provides an optimized complement to vascular quantity in prognosis of postoperative patients.
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影响因子：
50.3
作者：
Cooke VG;LeBleu VS;Keskin D;Khan Z;O'Connell JT;Teng Y;Duncan MB;Xie L;Maeda G;Vong S;Sugimoto H;Rocha RM;Damascena A;Brentani RR;Kalluri R
通讯作者：
Kalluri R
影响因子：
3.4
作者：
Jia, Jin-Bin;Wang, Wen-Quan;Tang, Zhao-You
通讯作者：
Tang, Zhao-You
分子机制和血管生成的临床应用。
影响因子：
64.8
作者：
Carmeliet, Peter;Jain, Rakesh K.
通讯作者：
Jain, Rakesh K.
影响因子：
33.6
作者：
Goel S;Duda DG;Xu L;Munn LL;Boucher Y;Fukumura D;Jain RK
通讯作者：
Jain RK
影响因子：
11.2
作者：
Hezel AF;Deshpande V;Zimmerman SM;Contino G;Alagesan B;O'Dell MR;Rivera LB;Harper J;Lonning S;Brekken RA;Bardeesy N
通讯作者：
Bardeesy N