An Indexed Genomic Library for Paramecium Complementation Cloning

An Indexed Genomic Library for Paramecium Complementation Cloning
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用于草履虫互补克隆的索引基因组文库

DOI:
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发表时间:
2000
影响因子:
2.2
通讯作者:
Jean Cohen
Jean Cohen
中科院分区:
生物学3区
文献类型:
--
作者:
A. Keller;Jean Cohen

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ABSTRACT. Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.
通过草履虫中的互补克隆基因。
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