The cloning by complementation of the pawn-A gene in Paramecium.
The cloning by complementation of the pawn-A gene in Paramecium.
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草履虫 pawn-A 基因的互补克隆。
DOI：
10.1093/genetics/149.2.947
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Kung,C
中科院分区：
文献类型：
--
作者：
Haynes,WJ;Vaillant,B;Preston,RR;Saimi,Y;Kung,C
The genetic dissection of a simple avoidance reaction behavior in Paramecium tetraurelia has shown that ion channels are a critical molecular element in signal transduction. Pawn mutants, for example, were originally selected for their inability to swim backward, a trait that has since been shown to result from the loss of a voltage-dependent calcium current. The several genes defined by this phenotype were anticipated to be difficult to clone since the 800-ploid somatic macronucleus of P. tetraurelia is a formidable obstacle to cloning by complementation. Nonetheless, when the macronucleus of a pawn mutant (pwA/pwA) was injected with total wild-type DNA or a fractional library of DNA, its clonal descendants all responded to stimuli like the wild type. By sorting a fractional library, we cloned and sequenced a 2.3-kb fragment that restores the Ca2+current and excitability missing in pawn-A. Data from RNase protection assays, followed by the sequencing of mutant alleles and cDNA clones, established an open reading frame. The conceptually translated product suggests a novel protein that may be glycophosphatidylinositol anchored. We also discuss the general usefulness of this method in cloning other unknown DNA sequences from Paramecium that are functionally responsible for various mutant phenotypes.
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通过草履虫中的互补克隆基因。
DOI：
10.3109/01677069609107064
发表时间：
1996
期刊：
Journal of neurogenetics.
影响因子：
--
作者：
Haynes,WJ;Ling,KY;Saimi,Y;Kung,C
通讯作者：
Kung,C
四尿草履虫中补充 Ca2 通道突变的细胞质因子的表征。
影响因子：
1.9
作者：
N. Haga;M. Forte;Y. Saimi;C. Kung
通讯作者：
C. Kung
影响因子：
64.8
作者：
Atsuo Ogura;Kei Takahashi
通讯作者：
Kei Takahashi
影响因子：
64.8
作者：
Y. Satow;C. Kung
通讯作者：
C. Kung
四尿草履虫 pwA 组 pawn 突变体的膜电流。
影响因子：
2.8
作者：
Y. Satow;C. Kung
通讯作者：
C. Kung