Lung vitamin E transport processes are affected by both age and environmental oxidants in mice.
Lung vitamin E transport processes are affected by both age and environmental oxidants in mice.
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小鼠肺部维生素 E 转运过程受到年龄和环境氧化剂的影响。
DOI：
10.1016/j.taap.2007.04.010
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发表时间：
2007
影响因子：
3.8
通讯作者：
Davis,PaulA
中科院分区：
文献类型：
--
作者：
Valacchi,Giuseppe;Vasu,VihasT;Yokohama,Wallace;Corbacho,AnaM;Phung,Anh;Lim,Yunsook;Aung,HninHnin;Cross,CarrollE;Davis,PaulA
尽管α -生育酚（AT）具有重要的生理作用，但维持细胞和组织生育酚水平的分子机制仍有待充分研究。清除受体B1 （SRB1）是清除受体大家族中的一员，已被证明可通过载脂蛋白a -1介导的过程促进AT从HDL转移到外周组织，并在将AT输送到肺细胞中起重要作用。在目前的研究中，评估了年龄和两种环境氧化剂臭氧（O3） （0.25 ppm 6小时/天）和香烟烟雾（CS） （60 mg/m36小时/天）持续4天对小鼠肺组织中AT运输的选定方面的影响。老年小鼠血浆和肺组织AT水平分别比年轻小鼠高25% （p<0.05）和15% (p<0.05)，急性环境暴露于所使用剂量的o3或CS没有影响。RT-PCR检测AT转运蛋白（ATTP）、SRB1、CD36、ATP结合盒3 （ABCA3）和ABCA1的基因表达水平，Western blots检测SRB1、ATTP和ABCA1的蛋白表达水平。老年小鼠肺中ATTP、ABCA3、SRB1水平降低，CD36、ABCA1水平升高。急性暴露于o3或CS诱导老年和年轻小鼠肺中ATTP和SRB1的下降。暴露于o3和CS后，年轻和老年小鼠肺中的CD36均增加。这些发现表明，年龄和环境氧化剂暴露都会影响与肺AT稳态相关的途径，并以有利于肺AT下降的方式进行。然而，鉴于所采取的方法，这些影响不能追溯到这些途径的变化或任何特定肺相关细胞类型中AT含量的变化，因此强调需要进一步研究特定肺相关细胞类型。
Despite the physiological importance of alpha-tocopherol (AT), the molecular mechanisms involved in maintaining cellular and tissue tocopherol levels remain to be fully characterized. Scavenger receptor B1 (SRB1), one of a large family of scavenger receptors, has been shown to facilitate AT transfer from HDL to peripheral tissues via apo A-1-mediated processes and to be important in the delivery of AT to the lung cells. In the present studies the effects of age and two environmental oxidants ozone (O3) (0.25 ppm 6 h/day) and cigarette smoke (CS) (60 mg/m36 h/day) for 4 days on selected aspects of AT transport in murine lung tissues were assessed. While AT levels were 25% higher (p<0.05) and 15% lower (p<0.05) in plasma and lung tissue, respectively, in aged versus young mice, acute environmental exposure to O3or CS at the doses used had no effect. Gene expression levels, determined by RT-PCR of AT transport protein (ATTP), SRB1, CD36, ATP binding cassette 3 (ABCA3) and ABCA1 and protein levels, determined by Western blots for SRB1, ATTP and ABCA1 were assessed. Aged mouse lung showed a lower levels of ATTP, ABCA3 and SRB1 and a higher level CD36 and ABCA1. Acute exposure to either O3or CS induced declines in ATTP and SRB1 in both aged and young mice lung. CD36 increased in both young and aged mice lung upon exposure to O3and CS. These findings suggest that both age and environmental oxidant exposure affect pathways related to lung AT homeostasis and do so in a way that favors declines in lung AT. However, given the approach taken, the effects cannot be traced to changes in these pathways or AT content in any specific lung associated cell type and thus highlight the need for further follow-up studies looking at specific lung associated cell types.
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