Ten emerging SARS-CoV-2 spike variants exhibit variable infectivity, animal tropism, and antibody neutralization.
Ten emerging SARS-CoV-2 spike variants exhibit variable infectivity, animal tropism, and antibody neutralization.
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DOI：
10.1038/s42003-021-02728-4
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发表时间：
2021-10-13
影响因子：
5.9
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhang L;Cui Z;Li Q;Wang B;Yu Y;Wu J;Nie J;Ding R;Wang H;Zhang Y;Liu S;Chen Z;He Y;Su X;Xu W;Huang W;Wang Y
新出现的SARS-CoV-2突变导致了几波COVID-19大流行。本文利用SARS-CoV-2假病毒研究了10种新出现的SARS-CoV-2变体b .1.1.298、B.1.1.7（Alpha）、B.1.351（Beta）、P.1（Gamma）、P.2（Zeta）、B.1.429（Epsilon）、B.1.525（Eta）、B.1.526-1（Iota）、B.1.526-2（Iota）、b .1.1.318以及受体结合域7种相应的单氨基酸突变的感染性和抗原性。结果表明，除B.1.1.298外，大多数SARS-CoV-2变种假病毒对人和猴细胞系的传染性均略有增强，其中对Calu-3细胞的感染性尤以B.1.351、B.1.525和B.1.526最强。携带K417N/T、N501Y或e484k的变异体感染过表达ace2的小鼠细胞的能力显著增加。furin、TMPRSS2和cathepsin L的活性对大多数变异均升高。RBD氨基酸突变包括K417T/N、L452R、Y453F、S477N、E484K和N501Y，导致13种单克隆抗体中的11种显著免疫逃逸。而对恢复期血清或疫苗诱导血清的中和抗性主要是由E484K突变引起的。B.1.1.7-和b .1.351感染患者的恢复期血清比其他SARS-CoV-2变体更能中和这些变体。我们的研究为治疗性抗体和疫苗提供了见解，并强调了E484K突变的重要性。张莉、崔志敏、李倩倩等人利用基于vsv的假病毒系统比较了10种SARS-CoV-2变异体的传染性、宿主亲和性和抗原性。他们的研究结果表明，携带E484K的变异对中和的敏感性降低最为显著，并可能为SARS-CoV-2感染的相关治疗方法的开发提供进一步的见解。
Emerging mutations in SARS-CoV-2 cause several waves of COVID-19 pandemic. Here we investigate the infectivity and antigenicity of ten emerging SARS-CoV-2 variants—B.1.1.298, B.1.1.7(Alpha), B.1.351(Beta), P.1(Gamma), P.2(Zeta), B.1.429(Epsilon), B.1.525(Eta), B.1.526-1(Iota), B.1.526-2(Iota), B.1.1.318—and seven corresponding single amino acid mutations in the receptor-binding domain using SARS-CoV-2 pseudovirus. The results indicate that the pseudovirus of most of the SARS-CoV-2 variants (except B.1.1.298) display slightly increased infectivity in human and monkey cell lines, especially B.1.351, B.1.525 and B.1.526 in Calu-3 cells. The K417N/T, N501Y, or E484K-carrying variants exhibit significantly increased abilities to infect mouse ACE2-overexpressing cells. The activities of furin, TMPRSS2, and cathepsin L are increased against most of the variants. RBD amino acid mutations comprising K417T/N, L452R, Y453F, S477N, E484K, and N501Y cause significant immune escape from 11 of 13 monoclonal antibodies. However, the resistance to neutralization by convalescent serum or vaccines elicited serum is mainly caused by the E484K mutation. The convalescent serum from B.1.1.7- and B.1.351-infected patients neutralized the variants themselves better than other SARS-CoV-2 variants. Our study provides insights regarding therapeutic antibodies and vaccines, and highlights the importance of E484K mutation. Li Zhang, Zhimin Cui, and Qianqian Li et al. compare the infectivity, host tropism, and antigenicity of 10 SARS-CoV-2 variants using a VSV-based pseudovirus system. Their results suggest that variants carrying E484K display the most significant reduction in sensitivity to neutralization, and may provide further insight into the development of relevant therapeutics for SARS-CoV-2 infection.
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影响因子：
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影响因子：
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10.1016/s0140-6736(21)00183-5
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DOI：
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