Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways.
Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways.
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DOI：
10.1186/1476-9255-8-16
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发表时间：
2011-07-07
期刊：
影响因子：
--
通讯作者：
Kim K
中科院分区：
文献类型：
--
作者：
Lee S;Shin S;Kim H;Han S;Kim K;Kwon J;Kwak JH;Lee CK;Ha NJ;Yim D;Kim K
牛蒡子苷是从连翘中提取的一种有效成分，具有抗炎、抗氧化、抗菌、抗病毒等作用。然而，一直缺乏关于其对免疫活性的影响的研究。本研究的目的是探讨牛蒡子苷在LPS诱导的巨噬细胞中的抗炎作用及其可能的机制。我们通过RT-PCR和蛋白质印迹分析研究了促炎细胞因子的mRNA和蛋白质水平，然后通过流式细胞仪分析表面分子的变化。牛蒡子苷剂量依赖性地减少NO和促炎细胞因子如IL-1β、IL-6、TNF-α和PGE 2的产生，并且分别通过RT-PCR和western blot分析确定其降低基因和蛋白水平。牛蒡子苷还能抑制B7-1和B7-2等共刺激分子的表达。牛蒡子苷还能抑制巨噬细胞核转录因子NF-κB的活化。综上所述，这些结果提供了牛蒡子苷在炎症性疾病中的生物活性的证据，并表明牛蒡子苷可能通过抑制促炎介质通过NF-kB的失活而发挥抗炎作用。
Arctiin, isolated from Forsythia suspensa has been reported to have anti-inflammatory, anti-oxidant, antibacterial, and antiviral effects in vitro. However, there has been a lack of studies regarding its effects on immunological activity. The aim of this study is to investigate the anti-inflammatory potential and possible mechanisms of arctiin in LPS-induced macrophages. We investigated the mRNA and protein levels of proinflammatory cytokines through RT-PCR and western blot analysis, followed by a FACS analysis for surface molecule changes. Arctiin dose dependently decreased the production of NO and proinflammatory cytokines such as IL-1β, IL-6, TNF-α, and PGE2, and it reduced the gene and protein levels as determined by RT-PCR and western blot analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 were also inhibited by arctiin. Furthermore, the activation of the nuclear transcription factor, NF-κB in macrophages was inhibited by arctiin. Taken together these results provide evidence of the bioactivity of arctiin in inflammatory diseases and suggest that arctiin may exert anti-inflammatory effect by inhibiting the pro-inflammatory mediators through the inactivation of NF-kB.
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