Mutants of Escherichia coli defective for replicative transposition of bacteriophage Mu.
Mutants of Escherichia coli defective for replicative transposition of bacteriophage Mu.
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噬菌体 Mu 复制转座缺陷的大肠杆菌突变体。
DOI：
10.1128/jb.167.3.905-919.1986
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发表时间：
1986
影响因子：
3.2
通讯作者：
Howe,MM
中科院分区：
文献类型：
--
作者：
Ross,W;Shore,SH;Howe,MM
我们分离了 142 个大肠杆菌 K-12 Mu cts Killysogen 的 Hir-（宿主复制抑制）突变体，该突变体在热诱导和 Mu 复制转座的杀伤作用中幸存下来。通过插入 Tn5 或 Tn10 的卡那霉素抗性衍生物诱导的所有 86 个突变以及大约三分之一的自发突变均通过 P1 转导发现与 zdh-201::Tn10 或 Tn10-1230 相关，表明它们分别位于 HimA 或髋部内或附近。对于这些突变的代表性组，通过携带himA+基因的质粒或通过lambda hip+转导噬菌体的互补分别证实了它们被鉴定为himA或hip突变。其余一些自发发生的突变位于 gyrA 或 gyrB（编码 DNA 旋转酶的基因）中。 gyrA 突变通过 P1 与 zei::Tn10 和 Nalr gyrA 等位基因的连锁来鉴定； gyrB 中的那些是通过与 tna::Tn10 和 gyrB(Ts) 等位基因的连锁来定义的。在携带这些 gyrA 或 gyrB 突变的菌株中，pBR322 质粒 DNA 表现出超螺旋水平的改变。 Mu cts 的生长程度在测试的各种旋转酶突变体中有所不同。一种gyrA突变体中的噬菌体产量严重减少，但在其他gyrA和gyrB突变体中，噬菌体产量仅延迟并略有减少。相反，Kil-Mu的生长在所有测试的旋转酶突变体宿主中都大大减少。
We isolated 142 Hir- (host inhibition of replication) mutants of an Escherichia coli K-12 Mu cts Kil- lysogen that survived heat induction and the killing effect of Mu replicative transposition. All the 86 mutations induced by insertion of Tn5 or a kanamycin-resistant derivative of Tn10 and approximately one-third of the spontaneous mutations were found by P1 transduction to be linked to either zdh-201::Tn10 or Tn10-1230, indicating their location in or near himA or hip, respectively. For a representative group of these mutations, complementation by a plasmid carrying the himA+ gene or by a lambda hip+ transducing phage confirmed their identification as himA or hip mutations, respectively. Some of the remaining spontaneously occurring mutations were located in gyrA or gyrB, the genes encoding DNA gyrase. Mutations in gyrA were identified by P1 linkage to zei::Tn10 and a Nalr gyrA allele; those in gyrB were defined by linkage to tna::Tn10 and to a gyrB(Ts) allele. In strains carrying these gyrA or gyrB mutations, pBR322 plasmid DNA exhibited altered levels of supercoiling. The extent of growth of Mu cts differed in the various gyrase mutants tested. Phage production in one gyrA mutant was severely reduced, but it was only delayed and slightly reduced in other gyrA and gyrB mutants. In contrast, growth of a Kil- Mu was greatly reduced in all gyrase mutant hosts tested.
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聚（ADP-核糖）参与哺乳动物细胞的辐射反应。
DOI：
10.1080/09553008414551891
发表时间：
1984
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
E. Ben
通讯作者：
E. Ben
影响因子：
3.4
作者：
OLEINICK, NL;EVANS, HH
通讯作者：
EVANS, HH
体外聚（ADP-核糖基）化反应对 DNA 聚合酶 α、DNA 聚合酶 β、末端脱氧核苷酸转移酶和 DNA 连接酶 II 的抑制
DOI：
10.1016/0006-291x(85)91644-4
发表时间：
1985
影响因子：
3.1
作者：
K. Yoshihara;A. Itaya;Yasuharu Tanaka;Y. Ohashi;Kimihiko Ito;H. Teraoka;K. Tsukada;A. Matsukage;T. Kamiya
通讯作者：
T. Kamiya
抑制剂研究是否证明了聚 (ADP-核糖) 在 DNA 修复中的作用？
影响因子：
3.4
作者：
Cleaver,JE;Milam,KM;Morgan,WF
通讯作者：
Morgan,WF
影响因子：
3.4
作者：
BERGER, NA
通讯作者：
BERGER, NA