Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference.
Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference.
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胰腺癌差异蛋白的蛋白质组学分析：稳定 RNA 干扰导致 MBD1 敲低的影响。
DOI：
10.1186/1471-2407-8-121
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发表时间：
2008-04-29
期刊：
影响因子：
3.8
通讯作者：
Bai C
中科院分区：
文献类型：
--
作者：
Liu C;Chen Y;Yu X;Jin C;Xu J;Long J;Ni Q;Fu D;Jin H;Bai C
甲基化CpG结合域蛋白1（Methyl-CpG binding domain protein 1，MBD 1）是一种基因转录抑制因子，可能参与肿瘤发生过程中抑癌基因的失活。MBD 1在人胰腺癌中的过表达已有报道。在这项研究中，我们建立了一个MBD 1敲低胰腺癌细胞系（BxPC-3）使用稳定的RNA干扰，比较蛋白质组的变化之间的控制和MBD 1敲低细胞使用二维凝胶电泳和质谱。我们确定了五种上调的蛋白质和九种下调的蛋白质。这些蛋白质大多与肿瘤的发生有关，有些是人类恶性肿瘤的预后标志物。这些差异蛋白可能与MBD 1的功能有关，为MBD 1在胰腺癌发生发展中的作用机制提供了新的思路。
Methyl-CpG binding domain protein 1 (MBD1), a suppressor of gene transcription, may be involved in inactivation of tumor suppressor genes during tumorigenesis. Over-expression of MBD1 has been reported in human pancreatic carcinomas. In this study, we established a MBD1-knock-down pancreatic cancer cell line (BxPC-3) using stable RNA interference, to compare the proteomic changes between control and MBD1-knock-down cells using two-dimensional gel electrophoresis and mass spectrometry. We identified five proteins that were up-regulated and nine proteins that were down-regulated. Most of the identified proteins are involved in tumorigenesis, some are prognostic biomarkers for human malignant tumors. Our data suggest that these differential proteins may be associated with the function of MBD1, and provide some insight into the functional mechanism of MBD1 in the development of pancreatic cancer.
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DOI：
10.1016/s0006-291x(03)00253-5
发表时间：
2003-03-21
影响因子：
3.1
作者：
Patra, SK;Patra, A;Dahiya, R
通讯作者：
Dahiya, R
影响因子：
11.5
作者：
Willipinski-Stapelfeldt, B;Riethdorf, S;Pantel, K
通讯作者：
Pantel, K
影响因子：
4.8
作者：
Gober, MD;Smith, CC;Aurelian, L
通讯作者：
Aurelian, L
DOI：
10.1016/s0006-291x(02)02921-2
发表时间：
2003-01-17
影响因子：
3.1
作者：
Boengler, K;Pipp, F;Deindl, E
通讯作者：
Deindl, E
DOI：
10.1073/pnas.78.6.3531
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
WANG, C;GOMER, RH;LAZARIDES, E
通讯作者：
LAZARIDES, E