Cell-specific transcriptional profiling reveals candidate mechanisms regulating development and function of uterine epithelia in mice.
Cell-specific transcriptional profiling reveals candidate mechanisms regulating development and function of uterine epithelia in mice.
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DOI：
10.1095/biolreprod.113.111971
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发表时间：
2013-10
影响因子：
3.6
通讯作者：
Spencer TE
中科院分区：
文献类型：
--
作者：
Filant J;Spencer TE
所有哺乳动物的子宫内膜都有腔上皮(LE)和腺上皮(GE)。LE介导着床的子宫容受性和胚泡附着，GE合成和分泌或运输与胚泡着床、子宫容受性和基质细胞蜕膜有关的生物活性物质。然而，调控子宫上皮细胞出生后发育的机制及其在成人中的功能尚不完全清楚。在这里，通过激光捕获显微解剖从出生后第10天(PD 10)和假孕2.5天和3.5天的子宫中分离出LE和Ge，进行了全面的基因芯片分析。这一数据与对含腺对照和非腺孕酮诱导的子宫腺基因敲除小鼠的子宫分析相结合，这些小鼠来自PD 10和DOPP 3.5。多种基因在两种上皮细胞中均有表达，但LE中的表达高于GE。在新生儿中，GE表达的基因丰富了形态发生、发育、迁移和维甲酸信号转导。在成人中，LE表达的基因在代谢过程和类固醇生物合成中得到丰富，而视黄醇信号、紧密连接、细胞外基质和激酶活性调节等基因在GE中得到丰富。上皮细胞的转录组差异支持这样的观点，即每种细胞类型在子宫中都有不同的和互补的功能。这里确定的候选基因和调控网络提供了一个框架，以发现调控出生后子宫上皮细胞发育的新机制及其在怀孕早期的功能。
All mammalian uteri have luminal (LE) and glandular epithelia (GE) in their endometrium. The LE mediates uterine receptivity and blastocyst attachment for implantation, and the GE synthesize and secrete or transport bioactive substances involved in blastocyst implantation, uterine receptivity, and stromal cell decidualization. However, the mechanisms governing uterine epithelial development after birth and their function in the adult are not fully understood. Here, comprehensive microarray analysis was conducted on LE and GE isolated by laser capture microdissection from uteri on Postnatal Day 10 (PD 10) and day of pseudopregnancy (DOPP) 2.5 and 3.5. This data was integrated with analysis of uteri from gland-containing control and aglandular progesterone-induced uterine gland knockout mice from PD 10 and DOPP 3.5. Many genes were expressed in both epithelia, but there was greater expression of genes in the LE than in the GE. In the neonate, GE-expressed genes were enriched for morphogenesis, development, migration, and retinoic acid signaling. In the adult, LE-expressed genes were enriched for metabolic processes and steroid biosynthesis, whereas retinoid signaling, tight junction, extracellular matrix, and regulation of kinase activity were enriched in the GE. The transcriptome differences in the epithelia support the idea that each cell type has a distinct and complementary function in the uterus. The candidate genes and regulatory networks identified here provide a framework to discover new mechanisms regulating development of epithelia in the postnatal uterus and their functions in early pregnancy.
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影响因子：
82.9
作者：
Cha J;Sun X;Dey SK
通讯作者：
Dey SK
影响因子：
3.8
作者：
Gray, CA;Burghardt, RC;Spencer, TE
通讯作者：
Spencer, TE
影响因子：
3.6
作者：
Hayashi, Kanako;Yoshioka, Shin;MacLean, James A., II
通讯作者：
MacLean, James A., II
DOI：
10.1530/rep-11-0114
发表时间：
2011-08
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Frolova AI;Moley KH
通讯作者：
Moley KH
影响因子：
4.8
作者：
Cheon, YP;Xu, XP;Bagchi, IC
通讯作者：
Bagchi, IC