Label-free enrichment of adrenal cortical progenitor cells using inertial microfluidics.
Label-free enrichment of adrenal cortical progenitor cells using inertial microfluidics.
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DOI：
10.1371/journal.pone.0046550
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Di Carlo D
中科院分区：
文献类型：
--
作者：
Hur SC;Brinckerhoff TZ;Walthers CM;Dunn JC;Di Carlo D
基于固有的生物物理细胞特性对活靶细胞进行被动和无标记分离，可以在分子生物标志物已知的应用中节省成本，并且有可能实现具有很少或没有已知分子生物标志物的细胞的分离。我们已经证明，利用单细胞和多细胞簇流过微通道时所经历的不同的流体动力惯性升力，从小鼠肾上腺的消化中纯化肾上腺皮质祖细胞。荧光染色以及基因表达测量证实，在不同出口收集的细胞群彼此不同。此外，通过该装置处理的原代小鼠细胞仍保持高度活力，并且可以在体外培养 10 天。所提出的靶细胞分离技术可以提供一种实用的方法来收集大量的存活完整细胞，以一种简单的无标记方式将干细胞生物学转化为再生医学。
Passive and label-free isolation of viable target cells based on intrinsic biophysical cellular properties would allow for cost savings in applications where molecular biomarkers are known as well as potentially enable the separation of cells with little-to-no known molecular biomarkers. We have demonstrated the purification of adrenal cortical progenitor cells from digestions of murine adrenal glands utilizing hydrodynamic inertial lift forces that single cells and multicellular clusters differentially experience as they flow through a microchannel. Fluorescence staining, along with gene expression measurements, confirmed that populations of cells collected in different outlets were distinct from one another. Furthermore, primary murine cells processed through the device remained highly viable and could be cultured for 10 days in vitro. The proposed target cell isolation technique can provide a practical means to collect significant quantities of viable intact cells required to translate stem cell biology to regenerative medicine in a simple label-free manner.
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