On the relationships in rhesus macaques between chronic ethanol consumption and the brain transcriptome.
On the relationships in rhesus macaques between chronic ethanol consumption and the brain transcriptome.
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DOI：
10.1111/adb.12501
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发表时间：
2018-01
影响因子：
3.4
通讯作者：
Hitzemann R
中科院分区：
文献类型：
--
作者：
Iancu OD;Colville A;Walter NAR;Darakjian P;Oberbeck DL;Daunais JB;Zheng CL;Searles RP;McWeeney SK;Grant KA;Hitzemann R
这是第一次描述慢性乙醇自我管理和非人类灵长类动物（恒河猴）的大脑转录组之间的关系。31只雄性动物每天自我给予乙醇，持续12个月以上。用RNA-Seq定量杏仁核中央核（CeA）和皮质区32中的基因转录。我们构建了共表达和共剪接网络，并确定了区域和网络类型之间的保留区域和差异区域。摄入量和转录之间的相关性包括在大脑区域以及表达和剪接之间的主要不同的基因集和注释类别;正相关和负相关也与不同的注释组相关。膜，突触和剪接注释类别中的模块（基因簇）丰富的正相关性（CeA），我们的共剪接分析进一步确定的基因只在外显子包含水平的影响。在CeA共表达网络中，我们在摄入相关的枢纽中鉴定了Rab 6 b，Cdk 18和Igsf 21，而在Area 32中，我们鉴定了一个不同的枢纽集，包括Ppp 3r 1和Myeov 2。总的来说，这些数据表明，过量的乙醇自我管理与广泛的表达和剪接机制，涉及膜和突触基因。
This is the first description of the relationship between chronic ethanol self-administration and the brain transcriptome in a non-human primate (rhesus macaque). Thirty-one male animals self-administered ethanol on a daily basis for over 12 months. Gene transcription was quantified with RNA-Seq in the central nucleus of the amygdala (CeA) and cortical Area 32. We constructed coexpression and cosplicing networks, and we identified areas of preservation and areas of differentiation between regions and network types. Correlations between intake and transcription included largely distinct gene sets and annotation categories across brain regions and between expression and splicing; positive and negative correlations were also associated with distinct annotation groups. Membrane, synaptic and splicing annotation categories were over-represented in the modules (gene clusters) enriched in positive correlations (CeA); our cosplicing analysis further identified the genes affected only at the exon inclusion level. In the CeA coexpression network, we identified Rab6b, Cdk18 and Igsf21 among the intake-correlated hubs, while in the Area 32, we identified a distinct hub set that included Ppp3r1 and Myeov2. Overall, the data illustrate that excessive ethanol self-administration is associated with broad expression and splicing mechanisms that involve membrane and synapse genes.
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使用WGCNA和壁炉架相关性的哺乳动物脑RNA-seq数据的固定网络分析。
影响因子：
3.7
作者：
Iancu OD;Colville A;Oberbeck D;Darakjian P;McWeeney SK;Hitzemann R
通讯作者：
Hitzemann R
DOI：
10.1111/gbb.12179
发表时间：
2014-11
期刊：
Genes, brain, and behavior
影响因子：
--
作者：
Enoch MA;Rosser AA;Zhou Z;Mash DC;Yuan Q;Goldman D
通讯作者：
Goldman D
DOI：
10.1111/j.1530-0277.2008.00866.x
发表时间：
2009-03-01
影响因子：
3.2
作者：
Bice, Paula;Valdar, William;Foroud, Tatiana
通讯作者：
Foroud, Tatiana
DOI：
10.1111/j.1530-0277.2012.01749.x
发表时间：
2012-08-01
影响因子：
3.2
作者：
Barkley-Levenson, Amanda M.;Crabbe, John C.
通讯作者：
Crabbe, John C.
猴子中的慢性酒精自我给药显示长期数量/频率分类稳定性。
DOI：
10.1111/acer.12547
发表时间：
2014-11
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Baker EJ;Farro J;Gonzales S;Helms C;Grant KA
通讯作者：
Grant KA