Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA.
Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA.
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DOI：
10.1083/jcb.148.2.283
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发表时间：
2000-01-24
期刊：
影响因子：
--
通讯作者：
Bazett-Jones DP
中科院分区：
文献类型：
--
作者：
Boisvert FM;Hendzel MJ;Bazett-Jones DP
早幼粒细胞白血病（PML）核小体（也称为ND10、POD和Kr小体）参与肿瘤发生和病毒感染。据报道，该亚核结构域富含RNA，是新生RNA合成的位点，暗示其直接参与基因表达的调控。我们用分析透射电子显微镜的方法来确定结构和组成的PML核体和周围的核质。电子光谱成像（ESI）表明，PML核体的核心是一个致密的，基于蛋白质的结构，直径为250 nm，不含可检测到的核酸。虽然PML核体既不含染色质也不含新生RNA，但新合成的RNA与PML核体的外周相关，并在紧靠PML核体核心的核质的染色质耗尽区域内发现。我们进一步表明，RNA不积累在结构的蛋白质核心。我们的研究结果否定了PML核体是转录位点的假设，但支持PML核体可能有助于形成有利于特定基因表达的核环境的模型。
The promyelocytic leukemia (PML) nuclear body (also referred to as ND10, POD, and Kr body) is involved in oncogenesis and viral infection. This subnuclear domain has been reported to be rich in RNA and a site of nascent RNA synthesis, implicating its direct involvement in the regulation of gene expression. We used an analytical transmission electron microscopic method to determine the structure and composition of PML nuclear bodies and the surrounding nucleoplasm. Electron spectroscopic imaging (ESI) demonstrates that the core of the PML nuclear body is a dense, protein-based structure, 250 nm in diameter, which does not contain detectable nucleic acid. Although PML nuclear bodies contain neither chromatin nor nascent RNA, newly synthesized RNA is associated with the periphery of the PML nuclear body, and is found within the chromatin-depleted region of the nucleoplasm immediately surrounding the core of the PML nuclear body. We further show that the RNA does not accumulate in the protein core of the structure. Our results dismiss the hypothesis that the PML nuclear body is a site of transcription, but support the model in which the PML nuclear body may contribute to the formation of a favorable nuclear environment for the expression of specific genes.
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DOI：
10.1083/jcb.112.5.785
发表时间：
1991-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ascoli CA;Maul GG
通讯作者：
Maul GG
核域10（ND10）的外围作为DNA病毒沉积部位。
影响因子：
7.8
作者：
Ishov, AM;Maul, GG
通讯作者：
Maul, GG
DOI：
10.1111/j.1432-1033.1996.0554z.x
发表时间：
1996-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Grotzinger, T;Sternsdorf, T;Will, H
通讯作者：
Will, H
影响因子：
3.3
作者：
Hendzel, MJ;Kruhlak, MJ;Bazett-Jones, DP
通讯作者：
Bazett-Jones, DP
影响因子：
2.4
作者：
Bazett-Jones, DP;Hendzel, MJ;Kruhlak, MJ
通讯作者：
Kruhlak, MJ