The periphery of nuclear domain 10 (ND10) as site of DNA virus deposition.
The periphery of nuclear domain 10 (ND10) as site of DNA virus deposition.
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核域10（ND10）的外围作为DNA病毒沉积部位。
DOI：
10.1083/jcb.134.4.815
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发表时间：
1996-08
影响因子：
7.8
通讯作者：
Maul, GG
中科院分区：
文献类型：
--
作者：
Ishov, AM;Maul, GG
DNA病毒进入细胞核后，它们启动转录级联反应，随后进行复制。我们调查了这些过程是发生在特定的核站点，还是像进入模式所暗示的那样，随机发生在整个核中。用核因子-1位点、卷曲小体和核区10(ND10)三个不同的核区作为标记，研究DNA病毒复制位点的相对位置。我们发现，在未感染的细胞中，这三个核结构域都具有很高的空间相关性。腺病毒感染后，发现核因子1和卷曲小体与某些病毒复制域相关。猴病毒40在ND10附近开始复制，但腺病毒5和1型单纯疱疹病毒在复制前修改了ND10。腺病毒E4orf 3基因缺失突变体保留ND10，并在ND10的外围开始复制。在单纯疱疹病毒1型即刻早期基因1突变体中也发现了同样的情况。通过发现输入的野生型病毒与ND10并列，证实了腺病毒5和疱疹病毒1型在ND10的沉积和复制不是突变表型。将病毒基因组运送到ND10不需要病毒基因的表达。因此，ND10的外围代表了至少三个DNA病毒家族的早期转录和复制发生的首选位置，提示了这个大的核结构域的一组新的功能特性。
After DNA viruses enter the nucleus, they initiate a transcriptional cascade which is followed by replication. We investigated whether these processes take place at specific nuclear sites or, as suggested by the mode of entry, randomly throughout the nucleus. Three distinct nuclear domains, nuclear factor-1 sites, coiled bodies, and nuclear domain 10 (ND10), were used as markers to investigate the relative position of DNA virus replication sites. We found that all three nuclear domains had a very high spatial correlation with each other in uninfected cells. After adenoviral infection, nuclear factor 1 and coiled bodies were found associated with some viral replication domains. Simian virus 40 begins replication adjacent to ND10 but adenovirus 5 and herpes simplex type 1 modified ND10s before replication. Adenovirus E4orf 3 gene deletion mutants retain ND10 and begin replication at the peripheries of ND10. The same was found for the herpes simplex virus type 1 immediate early gene 1 mutants. That the deposition and replication of adenovirus 5 and herpesvirus type 1 at ND10 was not a mutant phenotype was confirmed by finding the input wild-type virus juxtaposed to ND10. The transport of viral genomes to ND10 does not require viral gene expression. Thus, the peripheries of ND10 represent preferred sites where early steps of transcription and replication of at least three DNA virus families take place, suggesting a new set of functional properties for this large nuclear domain.
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影响因子：
7.8
作者：
CARVALHO, T;SEELER, JS;OHMAN, K;JORDAN, P;PETTERSSON, U;AKUSJARVI, G;CARMOFONSECA, M;DEJEAN, A
通讯作者：
DEJEAN, A
DOI：
10.1083/jcb.112.5.785
发表时间：
1991-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ascoli CA;Maul GG
通讯作者：
Maul GG
影响因子：
5.4
作者：
BOSHER, J;DAWSON, A;HAY, RT
通讯作者：
HAY, RT
影响因子：
3.7
作者：
BROUGH, DE;CLEGHON, V;KLESSIG, DF
通讯作者：
KLESSIG, DF
影响因子：
3.8
作者：
GRAHAM, FL;SMILEY, J;NAIRN, R
通讯作者：
NAIRN, R