Effects of Intermittent Mild Cold Stimulation on mRNA Expression of Immunoglobulins, Cytokines, and Toll-Like Receptors in the Small Intestine of Broilers.
Effects of Intermittent Mild Cold Stimulation on mRNA Expression of Immunoglobulins, Cytokines, and Toll-Like Receptors in the Small Intestine of Broilers.
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间歇轻度冷刺激对肉鸡小肠免疫球蛋白、细胞因子和Toll样受体mRNA表达的影响
DOI：
10.3390/ani10091492
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发表时间：
2020-08-24
期刊：
影响因子：
--
通讯作者：
Bao J
中科院分区：
文献类型：
--
作者：
Li S;Li J;Liu Y;Li C;Zhang R;Bao J
冷应激对肉鸡的健康和福利有不利影响。虽然一些研究表明，长期持续和温和的冷刺激可以提高免疫功能，但对间歇性冷刺激对肉鸡免疫调节的影响知之甚少。本研究在43 d内，每隔2 d对肉仔鸡进行低于正常饲养温度3 °C的冷刺激3 h和6 h，以探讨冷刺激对肉仔鸡肠道免疫功能的影响。研究结果证实，适当的轻度冷刺激对肉鸡肠道免疫有整体积极的影响。本研究中测试的轻度冷刺激具有成本效益，并且可能提高肉鸡的整体健康。摘要适当的冷刺激可提高肉仔鸡的免疫功能和应激耐受力。为探讨间歇性轻度冷刺激对肉仔鸡肠道免疫功能的影响，将240只1日龄健康Ross 308肉仔鸡随机分为3组：对照组（CC），在常规饲养环境温度下，每周逐渐降低3.5 ℃;组II（C3）和组III（C6），从第15天至第35天，每两天在低于CC中使用的温度3 °C下分别施加冷刺激3和6 h，并且从第35天至第43天在CC中使用的相同温度下施加冷刺激。在第22、29、35和43天，在十二指肠、空肠和回肠组织样品中研究免疫球蛋白（伊加和IgG）、细胞因子（IL 2、IL 6、IL 8、IL 17和IFNγ）和Toll样受体（TLR 2、TLR 4、TLR 5、TLR 7和TLR 21）的mRNA表达水平。从15 ~ 35日龄，IL 2和IFNγ mRNA在肉鸡肠道中的表达逐渐增加。在第43天冷刺激一周后，免疫球蛋白、细胞因子和Toll样受体（TLR）的mRNA水平稳定。总的来说，研究结果表明，比通常饲养温度低3 °C的冷刺激对肉鸡的肠道免疫力有积极影响。
Simple Summary Cold stress has been associated with adverse effects on health and welfare of broilers. Whilst several studies have shown that long-term sustained and mild cold stimulation can improve immune function, little is known of the effects of intermittent cold stimulation on immune modulation in broilers. In this study, broilers were submitted to cold stimulation of 3 °C below than the usual rearing temperature during 3 and 6 h every two days during 43 days to explore its effect on the intestinal immunity. The findings confirm that appropriate mild cold stimulation has an overall positive influence on the intestinal immunity of broilers. The mild cold stimulation tested in this study is cost-effective and likely enhances overall health of broilers. Abstract Appropriate cold stimulation can improve immune function and stress tolerance in broilers. In order to investigate the effect of intermittent mild cold stimulation on the intestinal immunity of broilers, 240 healthy one-day-old Ross 308 chickens were randomly divided into three groups: the control group (CC) housed in climatic chambers under usual rearing ambient temperature with a gradual 3.5 °C decrease per week; group II (C3) and group III (C6) to which cold stimulation at 3 °C below the temperature used in CC was applied every two days for 3 and 6 h, respectively, from day 15 to 35, and at the same temperature used in CC from day 35 to 43. The mRNA expression levels of immunoglobulins (IgA and IgG), cytokines (IL2, IL6, IL8, IL17, and IFNγ), and Toll-like receptors (TLR2, TLR4, TLR5, TLR7, and TLR21) were investigated in duodenum, jejunum, and ileum tissue samples on days 22, 29, 35, and 43. From day 15 to 35, mRNA expression of IL2 and IFNγ was increased in the intestine of broilers. After one week of cold stimulation on day 43, mRNA levels of immunoglobulins, cytokines, and Toll-like receptors (TLRs) stabilized. Collectively, the findings indicate that cold stimulation at 3 °C below the usual rearing temperature had a positive impact on intestinal immunity of broilers.
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