DNA topology and adaptation of Salmonella typhimurium to an intracellular environment.
DNA topology and adaptation of Salmonella typhimurium to an intracellular environment.
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DNA 拓扑结构和鼠伤寒沙门氏菌对细胞内环境的适应。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
C. Dorman
中科院分区：
文献类型：
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作者：
David G. Marshall;F. Bowe;Christine Hale;Gordon Dougan;C. Dorman
研究了兼性细胞内致病菌鼠伤寒沙门氏菌在适应J774A.1巨噬细胞样细胞内环境过程中DNA拓扑决定基因的表达。报告质粒被用来监测细胞内生长过程中DNA超螺旋的变化。检测到先前被证明在巨噬细胞中诱导的DPS和SPV基因的诱导，以及编码DNA旋转酶、整合宿主因子和类核相关蛋白H-NS的基因的表达。编码DNA松弛酶拓扑异构酶I的topA基因没有被诱导。报告质粒数据显示，巨噬细胞摄取鼠伤寒沙门氏菌后，细菌DNA变得松弛。这些数据表明，在适应细胞内环境的过程中，鼠伤寒沙门氏菌的DNA拓扑结构发生了显著的变化。讨论了描述这一过程如何运行的模型。
The expression of genes coding for determinants of DNA topology in the facultative intracellular pathogen Salmonella typhimurium was studied during adaptation by the bacteria to the intracellular environment of J774A.1 macrophage-like cells. A reporter plasmid was used to monitor changes in DNA supercoiling during intracellular growth. Induction of the dps and spv genes, previously shown to be induced in the macrophage, was detected, as was expression of genes coding for DNA gyrase, integration host factor and the nucleoid-associated protein H-NS. The topA gene, coding for the DNA relaxing enzyme topoisomerase I, was not induced. Reporter plasmid data showed that bacterial DNA became relaxed following uptake of S. typhimurium cells by the macrophage. These data indicate that DNA topology in S. typhimurium undergoes significant changes during adaptation to the intracellular environment. A model describing how this process may operate is discussed.
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影响因子：
15.9
作者：
Groisman, Eduardo A.
通讯作者：
Groisman, Eduardo A.
影响因子：
3.5
作者：
Cormack, BP;Valdivia, RH;Falkow, S
通讯作者：
Falkow, S
DOI：
10.1073/pnas.94.3.934
发表时间：
1997-02-04
影响因子：
11.1
作者：
Heithoff, DM;Conner, CP;Mahan, MJ
通讯作者：
Mahan, MJ
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影响因子：
10.5
作者：
Jones,BD
通讯作者：
Jones,BD
影响因子：
11.4
作者：
PARRALOPEZ, C;LIN, R;GROISMAN, EA
通讯作者：
GROISMAN, EA