Biases in Viral Metagenomics-Based Detection, Cataloguing and Quantification of Bacteriophage Genomes in Human Faeces, a Review.
Biases in Viral Metagenomics-Based Detection, Cataloguing and Quantification of Bacteriophage Genomes in Human Faeces, a Review.
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DOI：
10.3390/microorganisms9030524
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发表时间：
2021-03-04
期刊：
影响因子：
4.5
通讯作者：
Hill C
中科院分区：
文献类型：
--
作者：
Callanan J;Stockdale SR;Shkoporov A;Draper LA;Ross RP;Hill C
人类的肠道是大量微生物的聚居地，包括细菌、病毒、真菌和古生菌。虽然对这些微生物实体的兴趣主要集中在细菌成分上，但最近病毒成分引起了更多的关注。与传统的分离方法相比，宏基因组学的进步极大地增强了我们对人类微生物组（病毒组）中病毒的组成、多样性和功能的理解。我们强调病毒提取方法在鉴定和表征感染真核生物和细菌的病毒群落方面至关重要。不同的病毒提取方案，包括迄今为止一些最重要的人类病毒出版物中使用的方案，都引入了影响其总体结论的偏见。重要的是，应该在研究中明确强调方案的差异，最终目标是识别和承认与不同方案相关的偏差，并可能产生一种公正和标准化的方法来检查这部分人类微生物组。
The human gut is colonised by a vast array of microbes that include bacteria, viruses, fungi, and archaea. While interest in these microbial entities has largely focused on the bacterial constituents, recently the viral component has attracted more attention. Metagenomic advances, compared to classical isolation procedures, have greatly enhanced our understanding of the composition, diversity, and function of viruses in the human microbiome (virome). We highlight that viral extraction methodologies are crucial in terms of identifying and characterising communities of viruses infecting eukaryotes and bacteria. Different viral extraction protocols, including those used in some of the most significant human virome publications to date, have introduced biases affecting their a overall conclusions. It is important that protocol variations should be clearly highlighted across studies, with the ultimate goal of identifying and acknowledging biases associated with different protocols and, perhaps, the generation of an unbiased and standardised method for examining this portion of the human microbiome.
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影响因子：
15.5
作者：
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通讯作者：
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