Airway-Associated Macrophages in Homeostasis and Repair.
Airway-Associated Macrophages in Homeostasis and Repair.
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DOI：
10.1016/j.celrep.2020.108553
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发表时间：
2020-12-29
期刊：
影响因子：
8.8
通讯作者：
Rock JR
中科院分区：
文献类型：
--
作者：
Engler AE;Ysasi AB;Pihl RMF;Villacorta-Martin C;Heston HM;Richardson HMK;Thapa BR;Moniz NR;Belkina AC;Mazzilli SA;Rock JR
人们越来越认识到肺中髓系的异质性，但对与传导气道特异性相关的人群知之甚少。我们使用单细胞RNA测序，流式细胞术和免疫荧光来表征小鼠气管内稳态和上皮损伤/修复过程中的髓样细胞。我们鉴定了与肺间质巨噬细胞相似的粘膜下巨噬细胞和上皮内巨噬细胞。损伤后，中性粒细胞和粘膜下巨噬细胞（包括M2样巨噬细胞）早期增加。上皮内巨噬细胞在损伤后丢失，随后由CCR 2+单核细胞恢复。我们发现Ccr 2缺陷小鼠的气管上皮修复受损。肥大细胞和第2组先天性淋巴样细胞是白细胞介素-13（IL-13）的来源，IL-13刺激巨噬细胞并直接影响基底细胞的行为。它们与气道上皮的接近性使这些髓样细胞群成为气道疾病的潜在治疗靶点。Engler等人鉴定了与气管上皮相关的单核细胞和巨噬细胞的分子独特群体。损伤后气管髓样区室的组成发生变化，并且在Ccr 2+单核细胞不存在的情况下修复延迟。损伤相关的骨髓细胞和2型细胞因子直接影响基底细胞的行为。
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells of the mouse trachea during homeostasis and epithelial injury/repair. We identify submucosal macrophages, similar to lung interstitial macrophages, and intraepithelial macrophages. Following injury, there are early increases in neutrophils and submucosal macrophages, including M2-like macrophages. Intraepithelial macrophages are lost after injury and later restored by CCR2+ monocytes. We show that repair of the tracheal epithelium is impaired in Ccr2-deficient mice. Mast cells and group 2 innate lymphoid cells are sources of interleukin-13 (IL-13) that polarize macrophages and directly influence basal cell behaviors. Their proximity to the airway epithelium establishes these myeloid populations as potential therapeutic targets for airway disease. Engler et al. identify molecularly unique populations of monocytes and macrophages associated with the tracheal epithelium. The composition of the tracheal myeloid compartment changes upon injury, and repair is delayed in the absence of Ccr2+ monocytes. Injury-associated myeloid cells and type 2 cytokines directly affect basal cell behaviors.
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影响因子：
23.9
作者：
Lechner AJ;Driver IH;Lee J;Conroy CM;Nagle A;Locksley RM;Rock JR
通讯作者：
Rock JR
影响因子：
24.8
作者：
Ural BB;Yeung ST;Damani-Yokota P;Devlin JC;de Vries M;Vera-Licona P;Samji T;Sawai CM;Jang G;Perez OA;Pham Q;Maher L;Loke P;Dittmann M;Reizis B;Khanna KM
通讯作者：
Khanna KM
影响因子：
64.5
作者：
Levine JH;Simonds EF;Bendall SC;Davis KL;Amir el-AD;Tadmor MD;Litvin O;Fienberg HG;Jager A;Zunder ER;Finck R;Gedman AL;Radtke I;Downing JR;Pe'er D;Nolan GP
通讯作者：
Nolan GP
DOI：
10.1073/pnas.94.20.10838
发表时间：
1997-09-30
影响因子：
11.1
作者：
NobenTrauth, N;Shultz, LD;Paul, WE
通讯作者：
Paul, WE
影响因子：
16.6
作者：
Belkina, Anna C.;Ciccolella, Christopher O.;Snyder-Cappione, Jennifer E.
通讯作者：
Snyder-Cappione, Jennifer E.