Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin.
Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin.
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DOI：
10.1039/d2cb00173j
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发表时间：
2023-01-04
影响因子：
4.1
通讯作者：
Bugg, Timothy D. H.
中科院分区：
文献类型：
--
作者：
Alruwaili, Awatif;Rashid, Goran M. M.;Sodre, Victoria;Mason, James;Rehman, Zainab;Menakath, Anjali K.;Cheung, David;Brown, Steven P.;Bugg, Timothy D. H.
生物聚合物木质素被土壤微生物分解的途径可用于设计从木质素到可再生化学品的新生物催化途径，但目前尚未完全了解。为了探索这些途径，我们制备了侧链β-碳含有13 C的合成木质素。将[β-13 C]标记的DHP木质素喂入Rhodococcus jostii RHA 1中，导致13 C标记掺入代谢物草酸、4-羟基苯乙酸和4-羟基-3-甲氧基苯乙酸中，证实它们是由木质素分解产生的。我们已经鉴定了在约氏红球菌RHA 1中的乙醇酸氧化酶，其能够通过乙醇酸将乙醇醛氧化为草酸，从而鉴定了草酸形成的途径。R. Jostii乙醇酸氧化酶还催化4-羟基苯乙酸转化为4-羟基苯甲酰基甲酸，鉴定了另一种可能的途径为4-羟基苯甲酰基甲酸。从[β-13 C]-聚阿魏酸中也观察到标记草酸的形成，这为β-芳基醚单元的α，β-键断裂的自由基机理提供了实验证据。生物聚合物木质素被土壤微生物分解的途径可用于设计从木质素到可再生化学品的新生物催化途径，但目前尚未完全了解。
Pathways by which the biopolymer lignin is broken down by soil microbes could be used to engineer new biocatalytic routes from lignin to renewable chemicals, but are currently not fully understood. In order to probe these pathways, we have prepared synthetic lignins containing 13C at the sidechain β-carbon. Feeding of [β-13C]-labelled DHP lignin to Rhodococcus jostii RHA1 has led to the incorporation of 13C label into metabolites oxalic acid, 4-hydroxyphenylacetic acid, and 4-hydroxy-3-methoxyphenylacetic acid, confirming that they are derived from lignin breakdown. We have identified a glycolate oxidase enzyme in Rhodococcus jostii RHA1 which is able to oxidise glycolaldehyde via glycolic acid to oxalic acid, thereby identifying a pathway for the formation of oxalic acid. R. jostii glycolate oxidase also catalyses the conversion of 4-hydroxyphenylacetic acid to 4-hydroxybenzoylformic acid, identifying another possible pathway to 4-hydroxybenzoylformic acid. Formation of labelled oxalic acid was also observed from [β-13C]-polyferulic acid, which provides experimental evidence in favour of a radical mechanism for α,β-bond cleavage of β-aryl ether units. Pathways by which the biopolymer lignin is broken down by soil microbes could be used to engineer new biocatalytic routes from lignin to renewable chemicals, but are currently not fully understood.
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影响因子：
3.4
作者：
Euring, Markus;Trojanowski, Jerzy;Kharazipour, Alireza
通讯作者：
Kharazipour, Alireza
影响因子：
3.9
作者：
Rahmanpour, Rahman;Bugg, Timothy D. H.
通讯作者：
Bugg, Timothy D. H.
DOI：
10.1006/jmra.1994.1208
发表时间：
1994-10-01
期刊：
JOURNAL OF MAGNETIC RESONANCE SERIES A
影响因子：
--
作者：
METZ, G;WU, XL;SMITH, SO
通讯作者：
SMITH, SO
影响因子：
4.8
作者：
Han, Ying;Joosten, Henk-Jan;Dunaway-Mariano, Debra
通讯作者：
Dunaway-Mariano, Debra
影响因子：
2.9
作者：
Ahmad, Mark;Roberts, Joseph N.;Bugg, Timothy D. H.
通讯作者：
Bugg, Timothy D. H.