Immunofluorescence assay for serologic diagnosis of SARS.

Immunofluorescence assay for serologic diagnosis of SARS.
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DOI:
10.3201/eid1003.030493
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发表时间:
2004-03
影响因子:
11.8
通讯作者:
Tam JS
Tam JS
中科院分区:
医学2区
文献类型:
--
作者:
Chan PK;Ng KC;Chan RC;Lam RK;Chow VC;Hui M;Wu A;Lee N;Yap FH;Cheng FW;Sung JJ;Tam JS

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建立了一种检测SARS冠状病毒(SARS CoV)免疫球蛋白(IG)G抗体的间接免疫荧光法。所有确诊的SARS病例均表现出IgG抗体滴度的血清转化或四倍升高;无对照呈阳性。该方法的敏感性和特异性均为100%。免疫荧光检测可明确SARS冠状病毒感染状况。
We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
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