Continuous exposure to Plasmodium results in decreased susceptibility and transcriptomic divergence of the Anopheles gambiae immune system.
Continuous exposure to Plasmodium results in decreased susceptibility and transcriptomic divergence of the Anopheles gambiae immune system.
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DOI：
10.1186/1471-2164-8-451
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发表时间：
2007-12-05
期刊：
影响因子：
4.4
通讯作者：
Dimopoulos G
中科院分区：
文献类型：
--
作者：
Aguilar R;Das S;Dong Y;Dimopoulos G
疟原虫感染已被证明会损害蚊子媒介的适应性，减少其繁殖力和寿命。然而，从进化的角度来看，疟原虫感染作为一种选择性压力对蚊子的影响在很大程度上是未知的。在本研究中，我们讨论了连续感染伯氏疟原虫对冈比亚按蚊的感染抗性和全球基因表达的影响。冈比亚按蚊暴露于伯氏疟原虫感染的血液和感染16代后，对感染的易感性降低，约2.4%的蚊子总转录组的组成表达水平发生改变，免疫基因的基础表达水平降低，包括几种抗疟原虫因子。暴露于贝氏单胞杆菌的蚊群中，几种防御基因的感染反应性升高。我们的研究确定了寄生虫伯氏疟原虫对疟疾媒介冈比亚疟蚊施加的选择性压力的存在，这种压力导致蚊子对感染的容忍性降低，蚊子转录组调节的变化表明构成性免疫基因活性降低，但对疟原虫的攻击产生更强的免疫反应。
Plasmodium infection has been shown to compromise the fitness of the mosquito vector, reducing its fecundity and longevity. However, from an evolutionary perspective, the impact of Plasmodium infection as a selective pressure on the mosquito is largely unknown. In the present study we have addressed the effect of a continuous Plasmodium berghei infection on the resistance to infection and global gene expression in Anopheles gambiae. Exposure of A. gambiae to P. berghei-infected blood and infection for 16 generations resulted in a decreased susceptibility to infection, altered constitutive expression levels for approximately 2.4% of the mosquito's total transcriptome and a lower basal level of immune genes expression, including several anti-Plasmodium factors. The infection-responsiveness for several defense genes was elevated in the P. berghei exposed mosquito colonies. Our study establishes the existence of a selective pressure exerted by the parasite P. berghei on the malaria vector A. gambiae that results in a decreased permissiveness to infection and changes in the mosquito transcriptome regulation that suggest a decreased constitutive immune gene activity but a more potent immune response upon Plasmodium challenge.
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影响因子：
6.7
作者：
Dong Y;Aguilar R;Xi Z;Warr E;Mongin E;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
32.4
作者：
Frolet, Cecile;Thoma, Martine;Levashina, Elena A.
通讯作者：
Levashina, Elena A.
DOI：
10.1073/pnas.0404952102
发表时间：
2005-01-25
影响因子：
11.1
作者：
Choe, KM;Lee, H;Anderson, KV
通讯作者：
Anderson, KV
影响因子：
5.8
作者：
Ahmed, AM;Hurd, H
通讯作者：
Hurd, H
影响因子：
9.6
作者：
Ferguson, HM;Read, AF
通讯作者：
Read, AF